Chemical reagents for protein modification / Roger L. Lundblad.

Format:

Book

Author/Creator:

Lundblad, Roger L.

Language:

English

Subjects (All):

Proteins--Chemical modification.

Proteins.

Indicators and Reagents.

Proteins--analysis.

Medical Subjects:

Indicators and Reagents.

Proteins--analysis.

Physical Description:

339 pages : illustrations ; 25 cm

Edition:

Third edition.

Place of Publication:

Boca Raton, Fla. : CRC Press, [2005]

Summary:

Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, including protein microarrays, hydrogels, controlled-release pharmaceuticals, biotherapeutics, and proteomics.

The book analyzes individual amino acids, chemical cross-linking, and the chemical cleavage of peptide chains, as well as the general environmental factors that influence the reactivity of individual amino acid residues. It includes increased coverage of techniques for the characterization of modified proteins such as mass spectrometry, spectroscopic techniques, crystallographic analyses, nitrosylation reactions and other non-enzymatic modification processes, and the differential labeling of protein functional groups with reagents labeled with stable isotopes.

With more than 2000 references that describe the development of the various reagents, Chemical Reagents for Protein Modification, Third Edition offers extensive resources for researchers, including a list of current journals that publish site-specific chemical modification studies, a compilation of the most commonly used reagents and their properties, and a contact list of reagent suppliers.

Contents:

Chapter 1 The Site-Specific Chemical Modification of Proteins 1

Chapter 2 The Modification of Amino Groups 31

Chapter 3 The Modification of Histidine Residues 67

Chapter 4 The Modification of Arginine 95

Chapter 5 The Modification of Carboxyl Groups 123

Chapter 6 The Modification of Cysteine 139

Chapter 7 The Modification of Cystine 193

Appendix I Microprocedure for the Reduction and Carboxymethylation of Proteins 198

Materials 198

Procedure 199

Chapter 8 The Modification of Methionine 205

Chapter 9 The Modification of Tyrosine 213

Chapter 10 The Chemical Modification of Tryptophan 247

Chapter 11 The Chemical Cleavage of Peptide Bonds 269

Chapter 12 The Chemical Cross-Linking of Peptide Chains 285

Appendix I Some Reagents Used in the Modification of Proteins 301

Appendix II Assay of Protein Concentration in Solution 305

Microplate Biuret Assay 305

Reagent Preparation 305

Standard 305

Blank 306

Procedure 306

BCA (Bicinchoninic Acid) Assay for Protein 306

Reagent Preparation 306

Standard 306

Blank 307

Procedure 307

Dye-Binding Assay for Protein Using Coomassie Brilliant Blue G-250 307

Reagent Preparation 307

Standard 307

Blank 308

Procedure 308

Amino Acid Analysis 308.

Notes:

Includes bibliographical references and index.

ISBN:

0849319838

OCLC:

55616763