Neurons : methods and applications for the cell biologist / edited by Peter J. Hollenbeck, James R. Bamburg.

Format:

Book

Contributor:

Hollenbeck, Peter James.

Bamburg, James R.

Series:

Methods in cell biology 0091-679X ; v. 71.

Methods in cell biology, 0091-679X ; v. 71

Language:

English

Subjects (All):

Neurons.

Cytology.

Neurons--cytology.

Cell Culture Techniques--methods.

Cytological Techniques.

Medical Subjects:

Neurons--cytology.

Cell Culture Techniques--methods.

Cytological Techniques.

Physical Description:

xiii, 441 pages, 14 unnumbered pages : illustrations (some color) ; 25 cm.

Place of Publication:

San Diego : Academic Press, [2003]

Summary:

Many cell biologists study the properties of cells grown outside of the animal, in the controlled environment of a culture dish. Many cell biologists would benefit from directing their attention to nerve cells, but are afraid to try growing nerve cells in culture because it has the reputation for being much more difficult and unreliable than growing other kinds of cells. This volume is written by cell biologists and neuroscientists who are experts at growing various kinds of neurons in culture and using them for diverse experimental applications. The aim of this volume is to demystify the techniques used to grow nerve cells and to show cell biologists that it is something that they can easily master. * Contains culture methodology for more than a dozen types of CNS and PNS neurons * Includes most recent and reliable techniques from expert practitioners for specific experimental applications * Addresses the latest strategies for transfecting neurons

Contents:

1. Comparing the properties of neuronal culture systems: a shopping guide for the cell biologist / Peter J. Hollenbeck and James R. Bamburg

2. Growing and working with peripheral neurons / Yan He and Peter W. Baas

3. Dissection and culturing of chick ciliary ganglion neurons: a system well suited to synaptic study / Barbara W. Bernstein

4. The culture of chick forebrain neurons / Steven R. Heidemann ... [et al.]

5. Growing and working with spinal motor neurons / Thomas B. Kuhn

6. Avian purkinje neuronal cultures: extrinsic control of morphology by cell type and glutamate / Peter L. Jeffrey ... [et al.]

7. Culturing hippocampal and cortical neurons / Peter J. Meberg and Matthew W. Miller

8. Working with Xenopus spinal neurons in live cell culture / Timothy M. Gómez ... [et al.]

9. Culturing neurons from the snail Helisoma / Christopher S. Cohan, James L. Karnes, and Feng-Quan Zhou

10. The tibial-1 pioneer pathway: an in vivo model for neuronal outgrowth and guidance / Jennifer Bonner, Kimberly A. Gerrow, and Timothy P. O'Connor

11. Techniques to dissect cellular and subcellular function in the Drosophila nervous system / Heinrich J.G. Matthies and Kendal Broadie

12. PC12 cells as a model for studies of regulated secretion in neuronal and endocrine cells / T.F.J. Martin and R.N. Grishanin

13. B35 neuroblastoma cells: an easily transfected, cultured cell model of central nervous system neurons / Carol A. Otey, Malika Boukhelifa, and Patricia Maness

14. Live-cell imaging of slow axonal transport in cultured neurons / Anthony Brown

15. Making proteins into drugs: assisted delivery of proteins and peptides into living neurons / Gianluca Gallo

16. Transfection of primary central and peripheral nervous system neurons by electroporation / Cecilia Y. Martinez and Peter J. Hollenbeck

17. Biolistic transfection / Paul C. Bridgman, Michael E. Brown, and Irina Balan

18. Expression of transgenes in primary neurons from chick peripheral and central nervous systems by retroviral infection of early embryos / Peter J. Hollenbeck and D.M. Fekete

19. Production and use of replication-deficient adenovirus for transgene expression in neurons / L.S. Minamide ... [et al.].

Notes:

Includes bibliographical references and index.

ISBN:

0123525659

0123525667

OCLC:

52698581