Oligonucleotide synthesis : methods and applications / edited by Piet Herdewijn.

Format:

Book

Contributor:

Herdewijn, Piet.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 288.

Methods in molecular biology ; 288

Language:

English

Subjects (All):

Oligonucleotides--Synthesis--Laboratory manuals.

Oligonucleotides.

Oligonucleotides--chemical synthesis.

Oligonucleotides--Synthesis.

Oligonucleotides--chemistry.

Medical Subjects:

Oligonucleotides--chemical synthesis.

Oligonucleotides--chemistry.

Genre:

Laboratory manuals.

Physical Description:

xvi, 435 pages : illustrations ; 23 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2005]

Summary:

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Contents:

Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger ... [et al.]

RNA synthesis using 2'-o-(tert-butyldimethylsilyl) protection / Brian S. Sproat

RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / S. Hartsel... [et al.]

DTD; new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis

Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi

Assay for evaluating ribonuclease H-mediated degradation of RNA/antisense oligonucleotide duplexes / Annie Galarneau ... [et al.]

Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg

Solid-phase chemistry of circular oligonucleotides / Enrique Pedroso ... [et al.]

LNA synthesis / Henrik M. Pfundheller ... [et al.]

Synthesis of DNA mimics representing HypNa-pPNA heterooligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva

Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela ... [et al.]

Introduction of hypermodified nucleotides in rna / Darell Davis and Ashok C. Bajji

Chemical methods for peptide/oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait

Biotin labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho ... [et al.]

Universal labeling chemistry for nucleic acid detection on DNA-chips

Ali Laayoun ... [et al.]

Post-synthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun

Fluorescence based online detection as an analytical tool in RNA electrophoresis / Ute Scheffer and Michael Göbel

Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras

High thoughput production of optimized primers (fimers) for whole genome direct sequencing / Nikolai Polushin ... [et al.]

Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel

DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly ... [et al.]

Gene targeting using PNA (peptide nucleic acid) / Peter E. Nielsen

Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau

In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke

In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse ... [et al.]

Short double-stranded RNA (sirna) as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy.

Notes:

Includes bibliographical references and index.

ISBN:

1588292339

1592598234

OCLC:

54365526