Nitric oxide protocols.

Format:

Book

Contributor:

Hassid, Aviv.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 279.

Methods in molecular biology ; 279

Language:

English

Subjects (All):

Nitric oxide--Physiological effect--Laboratory manuals.

Nitric oxide.

Nitric-oxide synthase--Laboratory manuals.

Nitric-oxide synthase.

Nitric Oxide--analysis.

Nitric oxide--Physiological effect.

Nitric Oxide--physiology.

Nitric Oxide Synthase.

Medical Subjects:

Nitric Oxide--analysis.

Nitric Oxide--physiology.

Nitric Oxide Synthase.

Genre:

Laboratory manuals.

Physical Description:

xi, 246 pages : illustrations ; 24 cm.

Edition:

Second edition / edited by Aviv Hassid.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

A collection of cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues and fluids. These readily reproducible methods can be used to measure novel nitric oxide-related products such as protein nitration and nitrosation, as well as to express nitric oxide synthase in basic research and gene therapy using viral vectors.

Contents:

Measurement of nitric oxide by reconversion of nitrate/nitrite to NO / Reinhard Berkels, Svenja Purol-Schnabel, and Renate Roesen

High-resolution capillary electrophoresis of nitrite and nitrate in biological samples / Dmitri Y. Boudko

Nitrite and nitrate measurements in human urine by capillary electrophoresis / Edward Morcos and N. Peter Wiklund

In vivo nitric oxide measurements using a microaxial electrode / Yoshiichiro Kitamura ... [et al.]

Measurement of exhaled nitric oxide / Peter A. Steerenberg and Jan G.C. van Amsterdam

Nitric oxide imaging in neurons using confocal microscopy / Xiaoxiang Zheng ... [et al.]

Measurement of nitric oxide synthase activity in vivo and in vitro by gas Chromatography-mass spectroscopy / Dimitrios Tsikas

A nonradioactive assay for nitric oxide synthase activity in tissue extracts / Hazel Sutherland ... [et al.]

Measurement of nitric oxide-related enzymes in the brain by in situ hybridization / Olivier Braissant

Real-time polymerase chain reaction to quantify mRNA for endothelial nitric oxide synthase / Yi Chu and Frank M. Faraci

Dioxygen-dependent metabolism of nitric oxide / Paul R. Gardner, Anne M. Gardner, and Craig K. Hallstrom

Proteomic method for identification of tyrosine nitrated proteins / Kulwant S. Aulak ... [et al.]

Immunohistochemical detection of S-nitrosylated proteins / Andrew J. Gow ... [et al.]

Gene therapy techniques for the delivery of endothelial nitric oxide synthase (eNOS) to the corpus cavernosa for erectile dysfunction / Trinity J. Bivalacqua ... [et al.]

Adenovirus-mediated nitric oxide synthase gene transfer into the nucleus tractus solitarius in conscious rats / Yoshitaka Hirooka and Koji Sakai

Gene therapy in cancer via use of a retrovector having a tumor specificity and expressing inducible nitric oxide synthase / Masahide Kuroki

Gene transfer of nitric oxide synthase via the use of adeno-associated virus vectors / Yoshikazu Maeda, Kazuyuki Shimada, and Uichi Ikeda

Adenovirus-mediated nitric oxide synthase gene transfer / Kathleen G. Raman ... [et al.].

Notes:

Includes bibliographical references and index.

ISBN:

1588292371

OCLC:

53972540