Real-time PCR : an essential guide / edited by Kirstin Edwards, Julie Logan and Nick Saunders.

Format:

Book

Contributor:

Edwards, Kirstin (Kirstin J.)

Logan, Julie (Julie M. J.)

Saunders, Nick (Nick A.)

Language:

English

Subjects (All):

Polymerase chain reaction.

Polymerase Chain Reaction.

Medical Subjects:

Polymerase Chain Reaction.

Physical Description:

vi, 346 pages : illustrations ; 25 cm

Place of Publication:

Wymondham, Norfolk : Horizon bioscience, 2004.

Contents:

Chapter 1 An Introduction to Real-Time PCR / N.A. Saunders 1

Chapter 2 An Overview of Real-Time PCR Platforms / J.M.J. Logan, K.J. Edwards 13

Chapter 3 Homogeneous Fluorescent Chemistries for Real-Time PCR / M.A. Lee, D.J. Squirrell, D.L. Leslie, T. Brown 31

Chapter 4 Performing Real-Time PCR / K.J. Edwards 71

Chapter 5 Internal and External Controls for Reagent Validation / M.A. Lee, D.L. Leslie, D.J. Squirrell 85

Chapter 6 Quantitative Real-Time PCR / N.A. Saunders 103

Chapter 7 Analysis of mRNA Expression by Real-Time PCR / S.A. Bustin, T. Nolan 125

Chapter 8 Mutation Detection by Real-Time PCR / K.J. Edwards, J.M.J. Logan 185

Chapter 9 The Quantitative Amplification Refractory Mutation System / P. Punia, N.A. Saunders 211

Chapter 10 Real-Time NASBA / S. Hibbitts, J.D. Fox 229

Chapter 11 Applications of Real-Time PCR in Clinical Microbiology / A.D. Sails 247

Chapter 12 Application of Real-Time PCR to the Diagnosis of Invasive Fungal Infection / N. Isik, N.A. Saunders 327.

Notes:

Includes bibliographical references and index.

ISBN:

095452327X

OCLC:

55643610