Public health microbiology : methods and protocols / edited by John F.T. Spencer, Alicia L. Ragout de Spencer.

Format:

Book

Contributor:

Spencer, J. F. T.

Ragout de Spencer, Alicia L.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 268.

Methods in molecular biology, 1064-3745 ; v. 268

Language:

English

Subjects (All):

Diagnostic microbiology.

Environmental Microbiology.

Microbiological Techniques.

Medical Subjects:

Environmental Microbiology.

Microbiological Techniques.

Physical Description:

xvii, 548 pages ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

A collection of readily reproducible laboratory methods for the determination of various pathogenic microorganisms, their effects, and possible measures that can be taken to counter them. For bacterial and viral pathogens, the authors give special attention to PCR methods for detecting genes resistant to tetracycline, for resistance in Salmonella enterica, and for identifying and typing Campylobacter coli. For fungi the authors offer methods for computerized analysis and typing of fungal isolates, for the isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone. Other methods deal with hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, microbiological tests for sanitation equipment in factories, and the uses of animals in studies of various characteristics of lactobacilli. Review articles address the spread of pathogens from livestock and poultry production, including the threat of prion-based diseases.

Contents:

Part I Bacteria

1 Detection of Tetracycline Resistance Genes by PCR Methods / Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, Roderick I. Mackie 3

2 Integron Analysis and Genetic Mapping of Antimicrobial Resistance Genes in Salmonella enterica serotype Typhimurium / Mairead Daly, Seamus Fanning 15

3 Molecular-Based Identification and Typing of Campylobacter jejuni and C. coli / Brigid Lucey, Fiona O'Halloran, Seamus Fanning 33

4 Molecular Genotyping Methods and Computerized Analysis for the Study of Salmonella enterica / Ana Belen Vivanco, Juan Alvarez, Idoia Laconcha, Nuria Lopez-Molina, Aitor Rementeria, Javier Garaizar 49

Part II Viruses

5 Detection of Infectious Rotaviruses by Flow Cytometry / Albert Bosch, Rosa M. Pinto, Jaume Comas, Francesc-Xavier Abad 61

6 Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples / Kelly A. Reynolds 69

7 Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7 / Maite Muniesa, Juan Jofre 79

8 Molecular Genotyping of Irish Rotavirus Strains / Fiona O'Halloran, Seamus Fanning 89

9 Hepatitis A Virus: Molecular Detection and Typing / Gloria Sanchez, Cristina Villena, Albert Bosch, Rosa M. Pinto 103

Part III Fungi

10 Typing Fungal Isolates: Molecular Methods and Computerized Analysis / Aitor Rementeria, Ana Belen Vivanco, Ainara Cadaval, Maria Teresa Ruesga, Sonia Brena, Jose Ponton, Guillermo Quindos, Javier Garaizar 117

11 Fungal Isolation and Enumeration in Foods / Dante Javier Bueno, Julio Oscar Silva, Guillermo Oliver 127

12 Determination of Aflatoxins and Zearalenone in Different Culture Media / Dante Javier Bueno, Guillermo Oliver 133

Part IV Other Pathogens

13 Intracellular Multiplication of Legionella Species and the Influence of Amoebae on Their Intracellular Growth in Human Monocytes: Mono Mac 6 Cells and Acanthamoeba castellanii as Suitable In Vitro Models / Birgid Neumeister 141

14 Viability of Amoebae, Fungal Conidia, and Yeasts: Rapid Assessment by Flow Cytometry / Judith A. Noble-Wang, Shangtong Zhang, Daniel Price, Donald G. Ahearn 153

15 Detection and Differentiation of Cryptosporidium Oocysts in Water by PCR-RFLP / Lihua Xiao, Altaf A. Lal, Jianlin Jiang 163

16 Genotyping of Cryptosporidium parvum with Microsatellite Markers / Giovanni Widmer, Xiaochuan Feng, Sultan Tanriverdi 177

17 Immunomagnetic Separation of Pathogenic Organisms From Environmental Matrices / Gary P. Yakub, Kathleen L. Stadterman-Knauer 189

18 Detection of Erysipelothrix rhusiopathiae in Clinical and Environmental Samples / Silvana G. Fidalgo, Thomas V. Riley 199

19 Interaction Between Lactic Acid Bacteria and Gastrointestinal Nematodes of Caprine Origin / Diana Draksler, Maria Cecilia Monferran, Silvia Gonzalez 207

20 Molecular Detection of Genes Responsible for Cyanobacterial Toxin Production in the Genera Microcystis, Nodularia, and Cylindrospermopsis / Brendan P. Burns, Martin L. Saker, Michelle C. Moffitt, Brett A. Neilan 213

Part V Bacteriocins and Other Inhibitors

21 Purification of Antilisterial Bacteriocins / Jean-Marc Berjeaud, Yves Cenatiempo 225

22 The Hazard Analysis and Critical Control Point System in Food Safety / Anavella Gaitan Herrera 235

23 Testing Disinfectants in the Food Factory: Phenol Coefficient Method / Anavella Gaitan Herrera 281

24 Nontraditional Method of Evaluating Disinfectants: With Isolated Microorganisms From the Food Factory / Anavella Gaitan Herrera 289

25 Microbiological Analysis of Cosmetics / Anavella Gaitan Herrera 293

26 Helicobacter pylori and Food Products: A Public Health Problem / Anavella Gaitan Herrera 297

27 Microbiological Test for Sanitation of Equipment in the Food Factory / Anavella Gaitan Herrera 303

28 Spectrophotometric Determination of Histamine in Fisheries Using an Enzyme Immunoassay Method / Tania L. P. Pessatti, Jose D. Fontana, Marcos L. Pessatti 311

29 Flavonoids From Argentine Tagetes (Asteraceae) With Antimicrobial Activity / Maria L. Tereschuk, Mario D. Baigori, Lucia I. C. de Figueroa, Lidia R. Abdala 317

30 Purification of Bacteriocins Produced by Lactic Acid Bacteria / Lucila Saavedra, Patricia Castellano, Fernando Sesma 331

31 Production of Antimicrobial Substances by Lactic Acid Bacteria I: Determination of Hydrogen Peroxide / Maria Silvina Juarez Tomas, Maria Claudia Otero, Virginia Ocana, Maria Elena Nader-Macias 337

32 Production of Antimicrobial Substances by Lactic Acid Bacteria II: Screening Bacteriocin-Producing Strains With Probiotic Purposes and Characterization of a Lactobacillus Bacteriocin / Virginia S. Ocana, Maria Elena Nader-Macias 347

33 Statistical Models to Optimize Production of Probiotic Characteristics / Maria Silvina Juarez Tomas, Elena Bru, Maria Elena Nader-Macias 355

34 Meat-Model System Development for Antibacterial Activity Determination / Graciela Vignolo, Patricia Castellano 367

Part VI In Vivo Studies in Mice

35 Colonization Capability of Lactobacilli and Pathogens in the Respiratory Tract of Mice: Microbiological, Cytological, Structural, and Ultrastructural Studies / Rosa Cangemi de Gutierrez, Viviana M. Santos, Maria Elena Nader-Macias 373

36 Effects of Estrogen Administration on the Colonization Capability of Lactobacilli and Escherichia coli in the Urinary Tracts of Mice / Clara Silva, Rosario Rey, Maria Elena Nader-Macias 387

37 Effect of Lactobacilli Administration in the Vaginal Tract of Mice: Evaluation of Side Effects and Local Immune Response by Local Administration of Selected Strains / Elisa Vintini, Virginia Ocana, Maria Elena Nader-Macias 401

38 Determination of Bacterial Adhesion to Intestinal Mucus / Carlos Gusils, Vilma Morata, Silvia Gonzalez 411

39 Animal Model for In Vivo Evaluation of Cholesterol Reduction by Lactic Acid Bacteria / Maria Pia Taranto, Gabriela Perdigon, Marta Medici, Graciela Font de Valdez 417

40 Assessing Survival of Dairy Propionibacteria in Gastrointestinal Conditions and Adherence to Intestinal Epithelia / Gabriela Zarate, Silvia Gonzalez, Adriana Perez Chaia 423

Part VII Special Methods

41 Bacterial Surface Characteristics Applied to Selection of Probiotic Microorganisms / Maria Claudia Otero, Virginia S. Ocana, Maria Elena Nader-Macias 435

42 Adhesion Ability of Lactobacillus to Vaginal Epithelial Cells: Study by Microbiological Methods / Virginia S. Ocana, Maria Elena Nader-Macias 441

43 Hydroxylapatite Beads As an Experimental Model to Study the Adhesion of Lactic Acid Bacteria From the Oral Cavity to Hard Tissues / Maria del Carmen Ahumada Ostengo, Maria Elena Nader-Macias 447

44 Microtechnique for Identification of Lactic Acid Bacteria / Carlos Gusils, Adriana Perez Chaia, Guillermo Oliver, Silvia Gonzalez 453

45 Differentiation of Lactic Acid Bacteria Strains by Postelectrophoretic Detection of Esterases / Roxana Beatriz Medina, Marta Beatriz Katz, Silvia Gonzalez 459

46 Determination of Esterolytic and Lipolytic Activities of Lactic Acid Bacteria / Roxana Beatriz Medina, Marta Beatriz Katz, Silvia Gonzalez, Guillermo Oliver 465

47 Meat-Model System Development for Proteolytic Activity Determination / Graciela Vignolo, Silvina Fadda, Patricia Castellano, Cecilia Fontana 471

48 [beta]-Glucuronidase Method to Determine Mastitis Levels in Goat Milk / Ruben Oliszewski, Martha Nunez de Kairuz, Silvia Gonzalez, Guillermo Oliver 475

49 Differences Between Biogenic Amine Detection by HPLC Methods Using OPA and Dansyl Derivates / Maria R. Alberto, Mario E. Arena, Maria C. Manca de Nadra 481

50 Microflora of the Gastrointestinal Tract: A Review / Wei-Long Hao, Yuan-Kun Lee 491

51 Public Health Implications Related to Spread of Pathogens in Manure From Livestock and Poultry Operations / J. Lloyd Spencer, Jiewen Guan 503

52 Molecular Aspects of Disease Pathogenesis in the Transmissible Spongiform Encephalopathies / Suzette A. Priola, Ina Vorberg 517.

Notes:

Includes bibliographical references and index.

ISBN:

1588291170

OCLC:

53477897