Genomics, proteomics, and clinical bacteriology : methods and reviews / edited by Neil Woodford and Alan Johnson.

Format:

Book

Contributor:

Woodford, Neil.

Johnson, Alan P. (Alan Patrick), 1951-

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 266.

Methods in molecular biology ; 266

Language:

English

Subjects (All):

Molecular microbiology.

Bacterial genetics.

Genomics.

Proteomics.

Diagnostic bacteriology.

Genomics--methods.

Bacteriological Techniques--methods.

Proteomics--methods.

Medical Subjects:

Genomics--methods.

Bacteriological Techniques--methods.

Proteomics--methods.

Physical Description:

x, 395 pages ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

An accessible introduction to how genomics has and will provide novel methods for bacterial investigation and advance our understanding and knowledge of bacterial pathogenicity. The authors critically evaluate the applications of genomics to diagnostic bacteriology, highlighting both current and likely future uses, describing real-time PCR methods, and outlining the promise of microarrays in clinical bacteriology. Their discussion examines in detail genomic approaches to antibacterial discovery, the nature of pathogenicity, the discovery of new pathogens, the exploration of the concept of clonality in bacteria, and bacterial taxonomics.

Contents:

Part I Principles of Bacterial Genomics

1 Bacterial Genomes for the Masses: Relevance to the Clinical Laboratory / Mark J. Pallen 3

2 Public Databases: Retrieving and Manipulating Sequences for Beginners / Neil Woodford 17

3 Genome Sequencing and Annotation: An Overview / Makoto Kuroda, Keiichi Hiramatsu 29

4 Comparative Genomics: Digging for Data / Matthew B. Avison 47

5 Genome Plasticity: Insertion Sequence Elements, Transposons and Integrons, and DNA Rearrangement / Peter M. Bennett 71

6 Exploring and Exploiting Bacterial Proteomes / Stuart J. Cordwell 115

Part II Application of Genomics to Diagnostic Bacteriology

7 Molecular Diagnostics: Current Opinions / B. Cherie Millar, John E. Moore 139

8 Molecular Diagnostics: Future Probe-Based Strategies / Peter Marsh, Donald L. N. Cardy 167

9 Real-Time PCR / Nicholas A. Saunders 191

10 Microarrays for Bacterial Typing: Realistic Goal or Holy Grail? / Carola Van Ijperen, Nicholas A. Saunders 213

Part III Interrogating Bacterial Genomes

11 Genomic Approaches to Antibacterial Discovery / David J. Payne, Michael N. Gwynn, David J. Holmes, Marty Rosenberg 231

12 Using the Genome to Understand Pathogenicity / Dawn Field, Jennifer Hughes, E. Richard Moxon 261

13 Identification of Novel Pathogenicity Genes by PCR Signature-Tagged Mutagenesis and Related Technologies / Dario E. Lehoux, Francois Sanschagrin, Irena Kukavica-Ibrulj, Eric Potvin, Roger C. Levesque 289

14 Discovering New Pathogens: Culture-Resistant Bacteria / Andrew J. Lawson 305

15 Exploring the Concept of Clonality in Bacteria / Brian G. Spratt 323

16 Bacterial Taxonomics: Finding the Wood Through the Phylogenetic Trees / Robert J. Owen 353.

Notes:

Includes bibliographical references and index.

ISBN:

1588292185

OCLC:

54034861