Mobile genetic elements : protocols and genomic applications / edited by Wolfgang J. Miller and Pierre Capy.

Format:

Book

Contributor:

Miller, Wolfgang J.

Capy, Pierre.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 260.

Methods in molecular biology ; 260

Language:

English

Subjects (All):

Mobile genetic elements--Laboratory manuals.

Mobile genetic elements.

Interspersed Repetitive Sequences.

Genomics--methods.

Molecular Biology--methods.

Sequence Analysis--methods.

Medical Subjects:

Interspersed Repetitive Sequences.

Genomics--methods.

Molecular Biology--methods.

Sequence Analysis--methods.

Genre:

Laboratory manuals.

Physical Description:

xii, 289 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

Leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a wide range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts' genomic level, techniques for studying LTR retrotransposons in heterologous host systems, and mutagenesis protocols for investigating gene functions in a broad range of organisms. These cutting-edge methods offer investigators powerful genetic tools for dissecting the function of a specific gene, elaborating on the mechanisms leading to genetic change and diversity, and studying the evolutionary impact of mobile DNA on the biology and evolution of organisms.

Contents:

1 Mobile Genetic Elements as Natural Tools for Genome Evolution / Wolfgang J. Miller, Pierre Capy 1

2 Detection of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ Hybridization / Christian Biemont, Laurence Monti-Dedieu, Francoise Lemeunier 21

3 Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila / Patrizio Dimitri 29

4 Southern Blot Analysis of Individual Drosophila Flies / Nikolaj Junakovic 41

5 Computational Analysis of Transposable Element Sequences / I. King Jordan, Nathan J. Bowen 59

6 Biochemical Analysis of Long Terminal Repeat Retrotransposons / Andreas Bachmair, Karin Garber, Shin Takeda, Kazuhiko Sugimoto, Tetsuji Kakutani, Hirohiko Hirochika 73

7 Tn5 as a Molecular Genetics Tool: In Vitro Transposition and the Coupling of In Vitro Technologies With In Vivo Transposition / William S. Reznikoff, Igor Y. Goryshin, Jerry J. Jendrisak 83

8 Gene Targeting in Drosophila / Gregory B. Gloor 97

9 TE-Based Mutagenesis Systems in Plants: A Gene Family Approach / Michiel Vandenbussche, Tom Gerats 115

10 The Use of Double-Stranded RNA to Knock Down Specific Gene Activity / Mary K. Montgomery 129

11 The Application of LTR Retrotransposons as Molecular Markers in Plants / Alan H. Schulman, Andrew J. Flavell, T. H. Noel Ellis 145

12 MITE Display / Alexandra M. Casa, Alexander Nagel, Susan R. Wessler 175

13 Retroposon Mapping in Molecular Systematics / Norihiro Okada, Andrew M. Shedlock, Masato Nikaido 189

14 Transformation Systems in Insects / David A. O'Brochta, Peter W. Atkinson 227

15 Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: A Contemporary Review of Experimental Strategies / Zoltan Ivics, Zsuzsanna Izsvak 255.

Notes:

Includes bibliographical references and index.

ISBN:

1588290077

OCLC:

52895710