RNA interference, editing, and modification : methods and protocols / edited by Jonatha M. Gott.

Format:

Book

Contributor:

Gott, Jonatha M.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 265.

Methods in molecular biology, 1064-3745 ; 265

Language:

English

Subjects (All):

RNA editing--Laboratory manuals.

RNA editing.

RNA Editing.

RNA Interference.

Medical Subjects:

RNA Editing.

RNA Interference.

Genre:

Laboratory manuals.

Physical Description:

xiii, 435 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

A comprehensive collection of cutting-edge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification.

Contents:

Part I. RNA Interference and Gene Silencing

1 RNA Interference: Historical Overview and Significance / Mary K. Montgomery 3

2 Delivery of Double-Stranded RNA into Caenorhabditis elegans / Dawn Hull, Lisa Timmons 23

3 Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells / Amy A. Caudy, Gregory J. Hannon 59

4 Analysis of Gene Function in Trypanosoma brucei Using RNA Interference / Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, Elisabetta Ullu 73

5 Short Hairpin Activated Gene Silencing in Mammalian Cells / Patrick J. Paddison, Amy A. Caudy, Ravi Sachidanandam, Gregory J. Hannon 85

6 Geminivirus Vectors for Transient Gene Silencing in Plants / Nooduan Muangsan, Dominique Robertson 101

7 Posttranscriptional Gene Silencing in Plants / Susan Varsha Wesley, Chris Helliwell, Ming-Bo Wang, Peter Waterhouse 117

8 Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning / Victor Ambros, Rosalind C. Lee 131

Part II. RNA Editing and Modification

9 A Historical Perspective on RNA Editing: How the Peculiar and Bizarre Became Mainstream / Donna J. Koslowsky 161

10 Identification of Substrates for Adenosine Deaminases That Act on RNA / Daniel P. Morse 199

11 Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coli / Gillian M. Ring, Mary A. O'Connell, Liam P. Keegan 219

12 Isolation of an mRNA-Binding Protein Involved in C-to-U Editing / Carri A. Gerber, Anne Relich, Donna M. Driscoll 239

13 In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities / Kenneth Stuart, Reza Salavati, Robert P. Igo, Jr., Nancy Lewis Ernst, Setareh S. Palazzo, Bingbing Wang 251

14 Identification and Characterization of Trypanosome RNA-Editing Complex Components / Kenneth Stuart, Aswini K. Panigrahi, Achim Schnaufer 273

15 Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro / Elaine M. Byrne 293

16 Methods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellanii / Amanda J. Lohan, Michael W. Gray 315

17 In Vitro RNA Editing Systems From Higher Plant Chloroplasts / Tetsuro Hirose, Tetsuya Miyamoto, Junichi Obokata, Masahiro Sugiura 333

18 Studying RNA Editing in Transgenic Chloroplasts of Higher Plants / Ralph Bock 345

19 Detection and Quantification of Modified Nucleotides in RNA Using Thin-Layer Chromatography / Henri Grosjean, Gerard Keith, Louis Droogmans 357

20 Functional Characterization of 2'-O-Methylation and Pseudouridylation Guide RNAs / Tamas Kiss, Beata E. Jady 393

21 Experimental RNomics: A Global Approach to Identifying Small Nuclear RNAs and Their Targets in Different Model Organisms / Alexander Huttenhofer, Jerome Cavaille, Jean-Pierre Bachellerie 409.

Notes:

Includes bibliographical references and index.

ISBN:

1588292428

1592597750

OCLC:

53091023