Nucleic acid therapeutics in cancer / edited by Alan M. Gewirtz.

Format:

Book

Contributor:

Gewirtz, A. M. (Alan M.)

Series:

Cancer drug discovery and development

Language:

English

Subjects (All):

Antisense nucleic acids--Therapeutic use.

Cancer--Chemotherapy.

Neoplasms--therapy.

Antisense nucleic acids.

Genetic Therapy--methods.

Nucleic Acids--therapeutic use.

Medical Subjects:

Neoplasms--therapy.

Genetic Therapy--methods.

Nucleic Acids--therapeutic use.

Genre:

Conference papers and proceedings.

Physical Description:

x, 219 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

A cutting-edge review of the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting and clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically.

Contents:

Part I. Introduction and Perspective

1 Antisense Methodology: An Assessment After 25 Years / Fritz Eckstein 3

2 Nucleic Acid Therapeutics: An Introduction / Alan M. Gewirtz 15

Part II. Basic Methodology

3 Targeted Genome Modification Via Triple Helix Formation / Faye A. Rogers, Peter M. Glazer 27

4 Therapeutic Applications of Ribozymes / John J. Rossi 45

5 Use of Catalytic DNA in Target Validation and Therapeutics / Lun-Quan Sun 65

6 Targeted Destruction of Small, Stable RNAs: Principles Applicable to Antisense Therapies / David A. Dunbar, Susan J. Baserga 81

7 Mechanism of Action of Antisense RNA in Eukaryotic Cells / Zuo Zhang, Gordon G. Carmichael 89

Part III. Delivery

8 The Transport of Oligonucleotides Into Cells / R. L. Juliano 107

9 Peptide-Mediated Delivery of Antisense Oligonucleotides and Related Material / Eric Vives, Jean Philippe Richard, Bernard Lebleu 115

10 Molecular Vectors for Gene Delivery to Cancer Cells / Guy Zuber, Jean-Serge Remy, Patrick Erbacher, Pascale Belguise, Jean-Paul Behr 129

Part IV. Targeting

11 Considerations on the Design of Antisense Oligonucleotides / Rosel Kretschmer-Kazemi Far, Jens M. Warnecke, Georg Sczakiel 143

12 Identification of Hybridization Accessible Sequence in Messenger RNA / Lida K. Gifford, Ponzy Lu, Alan M. Gewirtz 155

Part V. Clinical Targets

13 Nucleic Acids As Gene Targeting Therapeutics / Joanna B. Opalinska, Susan E. Shetzline 167

14 LY900003 (Isis 3521) and G3139 (Genasense; Oblimersen): Phosphorothioate Antisense Oligonucleotides With Pleiotropic Mechanisms of Action / C. A. Stein, Nathalie Dias, Luba Benimetskaya, Jan S. Jepsen, Johnathan C. H. Lai, Anthony J. Raffo 177

15 Antisense Protein Kinase A-RI[alpha] Restores Normal Signal Transduction Signatures to Inhibit Tumor Growth / Yoon S. Cho-Chung 199.

Notes:

Papers from a conference held Apr. 2000 in Bryn Mawr, Pa.

Includes bibliographical references and index.

ISBN:

1588292584

OCLC:

53477911