Genetic recombination : reviews and protocols / edited by Alan S. Waldman.

Format:

Book

Contributor:

Waldman, Alan S.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 262.

Methods in molecular biology, 1064-3745 ; v. 262

Language:

English

Subjects (All):

Genetic recombination.

Recombination, Genetic.

Gene Expression.

Gene Targeting.

Genetic Engineering.

Medical Subjects:

Recombination, Genetic.

Gene Expression.

Gene Targeting.

Genetic Engineering.

Physical Description:

x, 260 pages : illustrations (color plate) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

Genetic recombination is any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. This book presents leading peer-reviewed protocols to carry out recombinant investigations in the lab. Part I provides approaches and model systems for studying several aspects of recombination in a variety of eukaryotic organisms and in mammalian parasites. Part II describes approaches for using recombination as a reporter of genomic instability in lower and higher eukaryotes. Part III discusses various methods and approaches for targeted genomic manipulation in higher and lower eukaryotes, while the final part presents biochemical analyses useful for furthering the understanding of recombination mechanisms, thus making this an invaluable book for both the novice and the established researcher in the field of recombination.

Contents:

Determination of mitotic recombination rates by fluctuation analysis in Saccharomyces cerevisiae / Rachelle Miller Spell and Sue Jinks-Robertson

Determination of intrachromosomal recombination rates in cultured mammalian cells / Jason A. Smith and Alan S. Waldman

Intrachromosomal homologous recombination in Arabidopsis thaliana / Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta

Analysis of recombinational repair of DNA double-strand breaks in mammalian cells with I-SceI nuclease / Jac A. Nickoloff and Mark A. Brenneman

Transformation of monomorphic and pleomorphic Trypanosoma brucei / Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry

Forward genetic screens for meiotic and mitotic recombination-defective mutants in mice / Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima

Detecting carcinogens with the yeast DEL assay / Richard J. Brennan and Robert H. Schiestl

In vivo DNA deletion assay to detect environmental and genetic predisposition to cancer / Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, and Robert H. Schiestl

Gene targeting at the chromosomal immunoglobulin locus: a model system for the study of mammalian homologous recombination mechanisms / Mark D. Baker

DNA fragment transplacement in Saccharomyces cerevisiae: some genetic considerations / Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis

Targeted gene modification using triplex-forming oligonucleotides / Jean Y. Kuan and Peter M. Glazer

Using nucleases to stimulate homologous recombination / Dana Carroll

Enhancement of in vivo targeted nucleotide exchange by nonspecific carrier DNA / Katie K. Maguire and Eric B. Kmiec

Chromatin immunoprecipitation to investigate protein-DNA interactions during genetic recombination / Tamara Goldfarb and Eric Alani

Holliday junction branch migration and resolution assays / Angelos Constantinou and Stephen C. West.

Notes:

Includes bibliographical references and index.

ISBN:

1588292363

OCLC:

53307757