Protein purification protocols.

Format:

Book

Contributor:

Cutler, Paul, 1962-

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 244.

Methods in molecular biology ; v. 244

Language:

English

Subjects (All):

Proteins--Purification--Laboratory manuals.

Proteins.

Proteins--isolation & purification.

Proteins--Purification.

Medical Subjects:

Proteins--isolation & purification.

Genre:

Laboratory manuals.

Physical Description:

xii, 484 pages : illustrations ; 26 cm.

Edition:

Second edition / edited by Paul Cutler.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

This new edition of Protein Purification Protocols (1996) completely updates the existing protocols to reflect recent advances and adds the enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins.

Contents:

1 General Strategies / Shawn Doonan, Paul Cutler 1

2 Preparation of Extracts From Animal Tissues / J. Mark Skehel 15

3 Protein Extraction From Plant Tissues / Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, Peter R. Shewry 21

4 Extraction of Recombinant Protein From Bacteria / Anne F. McGettrick, D. Margaret Worrall 29

5 Protein Extraction From Fungi / Paul D. Bridge, Tetsuo Kokubun, Monique S. J. Simmonds 37

6 Subcellular Fractionation of Animal Tissues / Norma M. Ryan 47

7 Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria / Alyson K. Tobin, Caroline G. Bowsher 53

8 The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds / William S. Pierpoint 65

9 Avoidance of Proteolysis in Extracts / Robert J. Beynon, Simon Oliver, Revised by Paul Cutler 75

10 Concentration of Extracts / Shawn Doonan 85

11 Making and Changing Buffers / Shawn Doonan 91

12 Purification and Concentration by Ultrafiltration / Paul Schratter, Revised by Paul Cutler 101

13 Bulk Purification by Fractional Precipitation / Shawn Doonan 117

14 Ion-Exchange Chromatography / Chris Selkirk 125

15 Hydrophobic Interaction Chromatography / Paul A. O'Farrell 133

16 Affinity Chromatography / Paul Cutler 139

17 Dye-Ligand Affinity Chromatography / Anne F. McGettrick, D. Margaret Worrall 151

18 Lectin Affinity Chromatography / Iris West, Owen Goldring, Revised by Paul Cutler 159

19 Immunoaffinity Chromatography / Paul Cutler 167

20 Immobilized Metal-Ion Affinity Chromatography / Tai-Tung Yip, T. William Hutchens, Revised by Paul Cutler 179

21 Chromatography on Hydroxyapatite / Shawn Doonan 191

22 Thiophilic Affinity Chromatography and Related Methods / Paul Matejtschuk 195

23 Affinity Precipitation Methods / Jane A. Irwin, Keith F. Tipton 205

24 Isoelectric Focusing / Reiner Westermeier 225

25 Chromatofocusing / Timothy J. Mantle, Patricia Noone 233

26 Size-Exclusion Chromatography / Paul Cutler 239

27 Fast Protein Liquid Chromatography / David Sheehan, Siobhan O'Sullivan 253

28 Reversed-Phase Chromatography of Proteins / William A. Neville 259

29 Extraction of Membrane Proteins / Kay Ohlendieck 283

30 Removal of Detergent From Protein Fractions / Kay Ohlendieck 295

31 Purification of Membrane Proteins / Kay Ohlendieck 301

32 Lyophilization of Proteins / Ciaran O'Fagain 309

33 Storage of Pure Proteins / Ciaran O'Fagain 323

34 Electroelution of Proteins From Polyacrylamide Gels / Michael J. Dunn 339

35 Electroblotting of Proteins From Polyacrylamide Gels / Michael J. Dunn 345

36 Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses / Neil A. Jones 353

37 Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis / Xun Zuo, David W. Speicher 361

38 Practical Column Chromatography / Shawn Doonan 377

39 Detection Methods / Jacek Mozdzanowski, Sudhir Burman 389

40 Peptide Proteomics / Dean E. McNulty, J. Randall Slemmon 411

41 Multidimensional Liquid Chromatography of Proteins / Rod Watson, Tim Nadler 425

42 Mass Spectrometry / David J. Bell 447

43 Purification of Therapeutic Proteins / Julian Bonnerjea 455

44 Purification Process Scale-Up / Karl Prince, Martin Smith 463.

Notes:

Includes bibliographical references and index.

ISBN:

1588290670

OCLC:

51936833