Chiral separations : methods and protocols / edited by Gerald Gübitz and Martin G. Schmid.

Format:

Book

Contributor:

Gübitz, Gerald.

Schmid, Martin G.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 243.

Methods in molecular biology ; 243

Language:

English

Subjects (All):

Enantiomers--Separation--Laboratory manuals.

Enantiomers.

Chirality.

Chromatography--methods.

Enantiomers--Separation.

Chromatography, High Pressure Liquid--methods.

Electrophoresis, Capillary--methods.

Chromatography, Thin Layer--methods.

Medical Subjects:

Chromatography--methods.

Chromatography, High Pressure Liquid--methods.

Electrophoresis, Capillary--methods.

Chromatography, Thin Layer--methods.

Genre:

Laboratory manuals.

Physical Description:

xiii, 432 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, NJ : Humana Press, [2004]

Summary:

Methods for enantiomer separation are very important for controlling synthesis, for checking race-mization, for enantiomeric purity control, and for pharmacokinetic studies. In Chiral Separations: Methods and Protocols, prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, and supercritical fluid chromatographic as well as recent electrochromotographic techniques. Each fully tested protocol includes an introduction to the principle underlying the method, equipment and reagent lists, and helpful notes on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Chiral Separations: Methods and Protocols will help both novice and advanced users not only choose optimal methods for their separation problems, but also readily obtain successful results.

Contents:

1 Chiral Separation Principles: An Introduction / Gerald Gubitz, Martin G. Schmid 1

2 Separation of Enantiomers by Thin-Layer Chromatography: An Overview / Kurt Gunther, Peter Richter, Klaus Moller 29

3 Cyclodextrin-Based Chiral Stationary Phases for Liquid Chromatography: A Twenty-Year Overview / Clifford R. Mitchell, Daniel W. Armstrong 61

4 Enantiomeric Separations by HPLC Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview / Tom Ling Xiao, Daniel W. Armstrong 113

5 Chiral Separation by HPLC Using Polysaccharide-Based Chiral Stationary Phases / Chiyo Yamamoto, Yoshio Okamoto 173

6 Applications of Polysaccharide-Based Chiral Stationary Phases for Resolution of Different Compound Classes / Hassan Y. Aboul-Enein, Imran Ali 183

7 Chiral Separation by HPLC With Pirkle-Type Chiral Stationary Phases / Myung Ho Hyun, Yoon Jae Cho 197

8 Chiral Separation by HPLC Using the Ligand-Exchange Principle / Vadim A. Davankov 207

9 Chiral Separations by HPLC Using Molecularly Imprinted Polymers / Peter Spegel, Lars I. Andersson, Staffan Nilsson 217

10 Indirect Enantioseparation by HPLC Using Chiral Benzofurazan-Bearing Reagents / Toshimasa Toyo'oka 231

11 Separation of the Racemic Trans-Stilbene Oxide by Sub-/Supercritical Fluid Chromatography / Leo Hsu, Genevieve Kennedy, Gerald Terfloth 247

12 Chiral Separations Using Macrocyclic Antibiotics in Capillary Electrophoresis / Timothy J. Ward, Colette M. Rabai 255

13 Enantioresolutions by Capillary Electrophoresis Using Glycopeptide Antibiotics / Salvatore Fanali 265

14 Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins / Wioleta Maruszak, Martin G. Schmid, Gerald Gubitz, Elzbieta Ekiert, Marek Trojanowicz 275

15 Chiral Separations by Capillary Electrophoresis Using Proteins as Chiral Selectors / Jun Haginaka 291

16 Cellulases as Chiral Selectors in Capillary Electrophoresis / Gunnar Johansson, Roland Isaksson, Goran Pettersson 307

17 Use of Chiral Crown Ethers in Capillary Electrophoresis / Martin G. Schmid, Gerald Gubitz 317

18 Chiral Separations by Capillary Electrophoresis Using Cinchona Alkaloid Derivatives as Chiral Counter-Ions / Michael Lammerhofer, Wolfgang Lindner 323

19 Chiral Separation by Capillary Electrophoresis Using Polysaccharides / Hiroyuki Nishi 343

20 Chiral Micellar Electrokinetic Chromatography / Koji Otsuka, Shigeru Terabe 355

21 Chiral Separation by Capillary Electrophoresis in Nonaqueous Medium / Marja-Liisa Riekkola, Heli Siren 365

22 Chiral Ligand-Exchange Capillary Electrophoresis and Capillary Electrochromatography / Martin G. Schmid, Gerald Gubitz 375

23 Enantioseparation in Capillary Chromatography and Capillary Electrochromatography Using Polysaccharide-Type Chiral Stationary Phases / Bezhan Chankvetadze 387

24 Chiral Separation by Capillary Electrochromatography Using Cyclodextrin Phases / Dorothee Wistuba, Jingwu Kang, Volker Schurig 401

25 Chiral Separations by Capillary Electrochromatography Using Molecularly Imprinted Polymers / Peter Spegel, Jakob Nilsson, Staffan Nilsson 411.

Notes:

Includes bibliographical references and index.

ISBN:

1588291502

OCLC:

51886500