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Molecular diagnosis of infectious diseases / edited by Jochen Decker, Udo Reischl.
LIBRA RC112 .M652 2004
Available from offsite location
- Format:
- Book
- Series:
- Methods in molecular medicine ; 94.
- Methods in molecular medicine ; 94
- Language:
- English
- Subjects (All):
- Communicable diseases--Molecular diagnosis--Laboratory manuals.
- Communicable diseases.
- Communicable Diseases--diagnosis.
- Biological Assay.
- Communicable Diseases--genetics.
- Immunologic Tests.
- Recombinant Proteins--diagnostic use.
- Molecular diagnosis.
- Medical Subjects:
- Communicable Diseases--diagnosis.
- Biological Assay.
- Communicable Diseases--genetics.
- Immunologic Tests.
- Recombinant Proteins--diagnostic use.
- Genre:
- Laboratory manuals.
- Physical Description:
- xv, 464 pages : illustrations (some color) ; 24 cm.
- Edition:
- Second edition.
- Place of Publication:
- Totowa, N.J. : Humana Press, [2004]
- Summary:
- This second edition of a classic laboratory manual describes cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors apply these new methodologies successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, as well as to diagnostic reagents in immunological assays.
- Contents:
- Part I. Protein-Based Identification of Antigens
- 1 Proteomic Approaches to Antigen Discovery / Karen M. Dobos, John S. Spencer, Ian M. Orme, John T. Belisle 3
- 2 Immunoproteomics / Alexander Krah, Peter R. Jungblut 19
- 3 Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins / Stephen Thompson 33
- Part II. DNA-Based Identification of Antigens
- 4 Representational Difference Analysis of cDNA / Lucas D. Bowler 49
- 5 Microarray Data Analysis and Mining / Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, Raffaele A. Calogero 67
- 6 Expression Cloning / Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, Yasir A. W. Skeiky 91
- Part III. Identification of Epitopes and Immunomodulatory Components
- 7 Determination of Epitopes by Mass Spectrometry / Christine Hager-Braun, Kenneth B. Tomer 109
- 8 Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays / Timothy W. Tobery, Michael J. Caulfield 121
- 9 Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity / Ludwig Deml, Jens Wild, Ralf Wagner 133
- 10 Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components / Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, Wulf Schneider-Brachert|p159
- Part IV. Expression of Recombinant Proteins
- 11 High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format / Jutta Drees, Jason Smith, Frank Schafer, Kerstin Steinert 179
- 12 Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins / Markus Fuhrmann 191
- 13 Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA / Marcus Graf, Ludwig Deml, Ralf Wagner 197
- Part V. Purification, Modification, and Renaturation of Recombinant Proteins
- 14 Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) / Udo Reischl 213
- 15 Purification of Recombinant Proteins with High Isoelectric Points / Raffaele A. Calogero, Anna Aulicino 225
- 16 Refolding of Inclusion Body Proteins / Marcus Mayer, Johannes Buchner 239
- 17 Small-Molecule-Protein Conjugation Procedures / Stephen Thompson 255
- Part VI. Characterization of Recombinant Proteins
- 18 Structural Characterization of Proteins and Peptides / Rainer Deutzmann 269
- 19 Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors / Claudia Hahnefeld, Stephan Drewianka, Friedrich W. Herberg 299
- 20 Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor / Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, Hans Wolf 321
- Part VII. Evaluation of Recombinant Proteins in Immunological Test Systems
- 21 Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays / John E. Butler 333
- 22 Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays / Alan Warnes, Anthony R. Fooks, John R. Stephenson 373
- 23 Basic Problems of Serological Laboratory Diagnosis / Walter Fierz 393
- 24 Molecular Diagnostics Resources on the Internet / Larry Winger 429
- Part VIII. Recombinant Receptor Molecules
- 25 Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells / Lars Toleikis, Olaf Broders, Stefan Dubel 447.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 1588292215
- OCLC:
- 52381702
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