Handbook of molecular and cellular methods in biology and medicine / edited by Leland J. Cseke ... [and others].

Format:

Book

Contributor:

Cseke, Leland J.

Language:

English

Subjects (All):

Molecular biology--Laboratory manuals.

Molecular biology.

Cytology--Laboratory manuals.

Cytology.

Molecular Biology.

Cytological Techniques.

Medical Subjects:

Molecular Biology.

Cytological Techniques.

Genre:

Laboratory manuals.

Physical Description:

580 pages : illustrations ; 26 cm

Edition:

Second edition.

Other Title:

Molecular and cellular methods in biology and medicine

Place of Publication:

Boca Raton : CRC Press, [2004]

Summary:

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has achieved several milestones. Reflecting these revolutionary advances, Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition provides you with an updated tool kit that documents conventional and modern approaches to tackling scientific research in the post-genomics era. With this book at hand, today's researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Contents:

Chapter 1. Isolation and Purification of DNA / Leland J. Cseke, William Wu, Chung-Jui Tsai 1

Chapter 2. Isolation and Purification of RNA / Chung-Jui Tsai, Leland J. Cseke, Scott A. Harding 25

Chapter 3. Extraction and Purification of Proteins / Soo Chul Chang, Leland J. Cseke 45

Chapter 4. Preparation of Nucleic Acid Probes / William Wu, Leland J. Cseke 67

Chapter 5. Southern Blot Hybridization / Chung-Jui Tsai, Leland J. Cseke 85

Chapter 6. Northern Blot Hybridization / Myeon Haeng Cho, Eun Kyeong Lee, Bin Goo Kang, Leland J. Cseke 105

Chapter 7. Preparation of Monoclonal and Polyclonal Antibodies against Specific Protein(s) / Leland J. Cseke, William Wu 113

Chapter 8. Western Blot Hybridization / Leland J. Cseke, William Wu, June Seung Lee, Soo Chul Chang 127

Chapter 9. cDNA Libraries / Leland J. Cseke, William Wu, Peter B. Kaufman 147

Chapter 10. Genomic DNA Libraries / Leland J. Cseke, William Wu, Peter B. Kaufman 193

Chapter 11. DNA Sequencing and Analysis / Leland J. Cseke, William Wu, Peter B. Kaufman 237

Chapter 12. DNA Site-Directed and Deletion Mutagenesis / Leland J. Cseke, William Wu 271

Chapter 13. DNA Footprinting and Gel-Retardation Assay / William Wu, Leland J. Cseke 291

Chapter 14. Differential Display / Darren H. Touchell, Yuh-Shuh Wang, Scott A. Harding, Chung-Jui Tsai 305

Chapter 15. Functional Genomics and DNA Microarray Technology / Gopi K. Podila, Chandrashakhar P. Joshi, Peter B. Kaufman 319

Chapter 16. In Vitro Translation of mRNA(s) and Analysis of Protein by Gel Electrophoresis / William Wu, Leland J. Cseke 347

Chapter 17. Plant Tissue and Cell Culture / Donghern Kim, Leland J. Cseke, Ara Kirakosyan, Peter B. Kaufman 357

Chapter 18. Gene Transfer and Expression in Animals / William Wu, Peter B. Kaufman, Leland J. Cseke 377

Chapter 19. Gene Transfer and Expression in Plants / William Wu, Leland J. Cseke, Peter B. Kaufman 409

Chapter 20. Inhibition of Gene Expression / Jaegal Shim, Junho Lee, Leland J. Cseke, William Wu 433

Chapter 21. Microscopy: Light, Scanning Electron, Environmental Scanning Electron, Transmission Electron, and Confocal / Casey R. Lu, Peter B. Kaufman, Leland J. Cseke 461

Chapter 22. Localization of Gene Expression / Scott A. Harding, Chung-Jui Tsai, Leland J. Cseke, Peter B. Kaufman, Soo Chul Chang, Feng Chen 487

Chapter 23. Bioseparation Techniques and Their Applications / Kefei Zheng, Daotian Fu, Leland J. Cseke, Ara Kirakosyan, Sara Warber, Peter B. Kaufman 509

Chapter 24. Combinatorial Techniques / Harry Brielmann 535

Chapter 25. Computational Data-Mining Methods Applied to Combinatorial Chemistry Libraries / Shirley Louise-May 549.

Notes:

Includes bibliographical references and index.

ISBN:

0849308151

OCLC:

52134773