Drosophila cytogenetics protocols / edited by Daryl S. Henderson.

Format:

Book

Contributor:

Henderson, Daryl S.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 247.

Methods in molecular biology ; 247

Language:

English

Subjects (All):

Drosophila melanogaster--Cytogenetics--Laboratory manuals.

Drosophila melanogaster.

Drosophila melanogaster--cytology.

Drosophila melanogaster--Cytogenetics.

Drosophila melanogaster--genetics.

Cell Division--genetics.

Cytogenetic Analysis--methods.

Medical Subjects:

Drosophila melanogaster--cytology.

Drosophila melanogaster--genetics.

Cell Division--genetics.

Cytogenetic Analysis--methods.

Genre:

Laboratory manuals.

Physical Description:

xiii, 468 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Contents:

1. The Chromosomes of Drosophila melanogaster / Daryl S. Henderson 1

2. Spermatogenesis: Analysis of Meiosis and Morphogenesis / Helen White-Cooper 45

3. Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy / Elena Rebollo, Cayetano Gonzalez 77

4. Immunocytological Analysis of Oogenesis / Endre Mathe 89

5. Cytological Analysis of Oogenesis / Seppo Nokkala, Christina Nokkala 129

6. Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants / Dmitry E. Koryakov, Natalia I. Mal'ceva, Robert C. King, Igor F. Zhimulev 139

7. Feulgen-DNA Cytophotometry for Estimating C Values / Ellen M. Rasch 163

8. Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary / Brian R. Calvi, Mary A. Lilly 203

9. Studying Nuclear Organization in Embryos Using Antibody Tools / Kristen M. Johansen, Jorgen Johansen 215

10. Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos / Daryl S. Henderson 235

11. Orcein Staining and the Identification of Polytene Chromosomes / John Tonzetich 249

12. Salivary Chromosome Analysis of Aberrations / Adelaide T. C. Carpenter 257

13. In Situ Hybridization to Polytene Chromosomes / Robert D. C. Saunders 279

14. Combined Immunostaining and FISH Analysis of Polytene Chromosomes / Sergey Lavrov, Jerome Dejardin, Giacomo Cavalli 289

15. Electron Microscopy of Polytene Chromosomes / Valery F. Semeshin, Elena S. Belyaeva, Victor V. Shloma, Igor F. Zhimulev 305

16. Analysis of Mitosis in Squash Preparations of Larval Brains: Orcein, Giemsa, Hoechst 33258, DAPI, Quinacrine, and N-Banding / Laura Fanti, Sergio Pimpinelli 325

17. Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo / Kami Ahmad, Kent G. Golic 333

18. Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling / Amy K. Csink 343

19. Immunostaining of Squash Preparations of Chromosomes of Larval Brains / Laura Fanti, Sergio Pimpinelli 353

20. Visualizing Mitosis in Whole-Mount Larval Brains / Daryl S. Henderson 363

21. Immunostaining of Whole-Mount Imaginal Discs / Brigitte de Saint Phalle 373

22. Wing Somatic Mutation and Recombination Test / Heloisa Helena Rodrigues de Andrade, Maria Luiza Reguly, Mauricio Lehmann 389

23. Analysis of Histoblasts / Mekkara Mandaravally Madhavan, Kornath Madhavan 413

24. Visualizing Apoptosis / Kimberly McCall, Jason S. Baum, Kristen Cullen, Jeanne S. Peterson 431

25. RNAi in Cultured Drosophila Cells / Ling-Rong Kao, Timothy L. Megraw 443.

Notes:

Includes bibliographical references and index.

ISBN:

1588290506

1592596657

OCLC:

52258074