Mammalian artificial chromosomes : methods and protocols / edited by Vittorio Sgaramella and Sandro Eridani.

Format:

Book

Contributor:

Sgaramella, V.

Eridani, Sandro.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 240.

Methods in molecular biology ; 240

Language:

English

Subjects (All):

Mammalian artificial chromosomes--Synthesis--Laboratory manuals.

Mammalian artificial chromosomes.

Chromosomes, Artificial, Mammalian.

Genetic Techniques.

Medical Subjects:

Chromosomes, Artificial, Mammalian.

Genetic Techniques.

Genre:

Laboratory manuals.

Physical Description:

xi, 269 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

Leading scientists in cell biology, genetics, cell culturing, and gene transfer present their most productive methods for the preparation, characterization, and use of artificial chromosomes. The authors cover all relevant areas of artificial chromosome research-from basic genetics to daring attempts at building new gene therapy tools-and describe possible applications in genetic manipulation requiring large portions of the genome. Each fully tested protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, reagent and equipment lists, and tips on troubleshooting and how to avoid pitfalls.

Contents:

1 From Natural to Artificial Chromosomes: An Overview / Vittorio Sgaramella, Sandro Eridani 1

2 Methodologies Applied to Domestic Animal Chromosomes / Leopoldo Iannuzzi 15

3 Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes / Grzegorz Ira, Jacek Krol, Jan Filipski 35

4 Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammals / Yuji Goto, Robert Feil 53

5 Centromeres and Neocentromeres / Gerard Roizes, Christoph Grunau, Jerome Buard, Albertina De Sario, Jacques Puechberty 77

6 Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins / Alessandro Vindigni, Gulnara Abdurashidova, Arturo Falaschi 105

7 Telomere Length Analysis and In Vitro Telomerase Assay / Fiorentina Ascenzioni, Pier Assunta Fradiani, Pierluigi Donini 123

8 Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice / Peter J. Hornsby 147

9 Chromosome-Based Vectors for Mammalian Cells: An Overview / Han N. Lim, Christine J. Farr 167

10 Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor / Jose I. de las Heras, Leonardo D'Aiuto, Howard Cooke 187

11 Use of Natural and Artificial Chromosome Vectors for Animal Transgenesis / Yoshimi Kuroiwa, Kazuma Tomizuka, Isao Ishida 207

12 Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique / Diane P. Monteith, Josephine D. Leung, Anita H. Borowski, Deborah O. Co, Tunde Praznovszky, Frank R. Jirik, Gyula Hadlaczky, Carl F. Perez 227

13 Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications / Michael R. Orford, Duangporn Jamsai, Samuel McLenachan, Panayiotis A. Ioannou 243.

Notes:

Includes bibliographical references and index.

ISBN:

1588290964

1592594344

OCLC:

52737516