Electric field-induced effects on neuronal cell biology accompanying dielectrophoretic trapping / T. Heida.

Format:

Book

Author/Creator:

Heida, T. (Tjitske), 1972-

Series:

Advances in anatomy, embryology, and cell biology 0301-5556 ; v. 173.

Advances in anatomy, embryology, and cell biology, 0301-5556 ; v. 173

Language:

English

Subjects (All):

Neurons.

Dielectrophoresis.

Nerves--Electric properties.

Nerves.

Microelectrodes.

Neurons--physiology.

Electromagnetic Fields.

Electrophysiology--methods.

Models, Neurological.

Signal Processing, Computer-Assisted.

Medical Subjects:

Neurons--physiology.

Electromagnetic Fields.

Electrophysiology--methods.

Models, Neurological.

Signal Processing, Computer-Assisted.

Physical Description:

ix, 80 pages : illustrations ; 24 cm.

Place of Publication:

Berlin ; New York : Springer, [2003]

Summary:

The concept of the cultured neuron probe was induced by the possible selective stimulation of nerves for functional recovery after a neural lesion or disease. The probe consists of a micro-electrode array on top of which groups of neuronal cells are cultured. An efficient method to position groups of neuronal cells on top of the stimulation sites of the micro-electrode array is developed. With negative dielectrophoretic forces, produced by non-uniform electric fields on polarizable particles, neuronal cells are trapped. Experimental results and model simulations describe the trapping process and its effect on neuronal cell viability.

Contents:

1.1 Neuro-Electronic Interfacing 1

1.1.1 Nervous System 1

1.1.2 Restoring Neuronal Functions 2

1.2 Culturing Neuronal Cells 3

1.2.1 Dissociation 3

1.2.2 Culturing Conditions 4

1.3 Positioning and Culturing Neuronal Cells on a Microelectrode Array 5

1.3.1 Microelectrode Array 5

1.3.2 Cell Positioning 6

1.4 Dielectrophoresis 7

1.4.1 Principle of Dielectrophoresis 7

1.4.2 Viability of Cells Exposed to Electric Fields 9

1.5 Scope of This Review 10

2 Dielectrophoretic Trapping of Neuronal Cells 11

2.1 Theory 11

2.1.1 Dielectrophoretic Force 11

2.1.2 Electrical Properties of Cells and Cell Suspensions 12

2.1.3 Modeling the Electrical Properties of a Suspended Biological Cell 13

2.2 Materials 14

2.2.1 Planar Quadrupole Microelectrode Structure 14

2.2.2 Electric Field Generation 16

2.2.3 Cells and Medium 17

2.2.3.1 First Series 17

2.2.3.2 Second Series 17

2.3 Theoretical Description of Dielectrophoretic Trapping 18

2.3.1 Estimation of the Dielectrophoretic Force 18

2.3.2 Electrode-Electrolyte Interface 20

2.3.3 Field-Induced Fluid Flow 21

2.3.4 Total Trapping Force 23

2.4 Experimental Description of Dielectrophoretic Trapping 23

2.4.1 Experimental Procedure 23

2.4.2 Temperature Rise in the Medium Due to the Electric Field 24

2.4.3 Experimental Results 24

2.4.3.1 Trapping Neurons Under Various Field Conditions 24

2.4.3.2 The Yield 25

2.4.3.3 Qualitative Aspects of Neuron Trapping 27

3 Exposing Neuronal Cells to Electric Fields 31

3.1 Theory 31

3.1.1 Membrane Breakdown 31

3.1.2 Pulse Length, Temperature, and Medium Conductivity Dependence 33

3.1.3 Pore Model 35

3.1.4 Electromechanical Model 37

3.1.5 Recovery of the Membrane 37

3.1.6 Methods of Observation 38

3.2 Theoretical Investigation of Induced Membrane Potentials of Neuronal Cells 38

3.2.1 The Model 38

3.2.2 Modeling Results 40

3.3 Experimental Investigation of Neuronal Membrane Breakdown 41

3.3.1 Experimental Procedure 41

3.3.2 Data Analysis Procedure 43

3.3.3 Experimental Results 43

4 Investigating Viability of Dielectrophoretically Trapped Neuronal Cells 47

4.1 Viability of Neuronal Cells Trapped at a High Frequency 47

4.1.1 Experimental Procedure 47

4.1.1.1 Experimental Setup 47

4.1.1.2 Data Analysis 48

4.1.2 Experimental Results 49

4.1.2.1 Number of Outgrowing and Nonoutgrowing Cortical Cells 49

4.1.2.2 Area of the Cortical Cells 51

4.1.2.3 Number of Processes 51

4.1.2.4 Process Length 51

4.1.2.5 Data Comparison 52

4.2 Viability of Neuronal Cells Trapped at Low Frequencies 54

4.2.1 Theoretical Estimation of the Maximum Membrane Potential 54

4.2.2 Experimental Procedure 55

4.2.2.1 Experimental Setup 55

4.2.2.2 Data Analysis 56

4.2.2.3 Contour Detection for Area Determination 56

4.2.2.4 Detection of Red and Green Stained Areas 56

4.2.3 Experimental Results 57

4.2.3.1 Total Area Covered with Cells 57

4.2.3.2 Staining of DEP-Trapped Cells 59

4.2.3.3 Adhesion in Relation to Field Strength and Frequency 61

4.2.3.4 Viability in Relation to Frequency 62

4.2.3.5 Cell Death 63

4.3 Recording Neuronal Activity 64

4.3.1 Extracellular Recording 64

4.3.2 MEA for DEP Trapping and Recording Neuronal Activity 65

4.3.3 Experimental Procedure 66

4.3.3.1 Experimental Setup 66

4.3.3.2 Spike Analysis 68

4.3.4 Recording Results 69.

Notes:

Includes bibliographical references (pages 73-77) and index.

ISBN:

3540006370

OCLC:

51804941