Pediatric hematology : methods and protocols / edited by Nicholas J. Goulden, Colin G. Steward.

Format:

Book

Contributor:

Goulden, Nicholas J.

Steward, Colin G.

Series:

Methods in molecular medicine ; 91.

Methods in molecular medicine ; 91

Language:

English

Subjects (All):

Pediatric hematology.

Medical protocols.

Hematologic Diseases.

Child.

Hematologic Diseases--diagnosis.

Infant.

Medical Subjects:

Hematologic Diseases.

Child.

Hematologic Diseases--diagnosis.

Infant.

Physical Description:

xii, 272 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cuttingedge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a gold-standard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders.

Contents:

Part I. Bone Marrow Failure Syndromes

1 Molecular Diagnosis of Fanconi Anemia and Dyskeratosis Congenita / Alex J. Tipping, Tom J. Vulliamy, Neil V. Morgan, Inderjeet Dokal 3

2 Molecular Diagnosis of Diamond-Blackfan Anemia / Sarah Ball, Karen Orfali 19

Part II. Red Blood Cell Disorders

3 Antenatal Diagnosis of Hemoglobinopathies / John M. Old 33

Part III. Platelet and Hemostatic Disorders

4 Prenatal Diagnosis of Hemophilia / David Stirling 65

5 Human Platelet Antigen Genotyping by PCR-SSP in Neonatal/Fetal Alloimmune Thrombocytopenia / Colin Hurd, Geoff Lucas 71

6 Multiplex PCR for the Detection of the Factor V Leiden and Prothrombin 20210A Mutations / Anne M. Sproul, Elizabeth A. Chalmers 79

Part IV. Immunodeficiency

7 Molecular Diagnosis of Congenital Immunodeficiency / David Eastwood, Kimberly C. Gilmour, Hubert B. Gaspar 91

Part V. Acute Leukemia

8 Molecular Techniques to Improve Outcome in Childhood ALL / Frederik W. van Delft, Vaskar Saha 111

9 Molecular Cytogenetics in Childhood Leukemia / Christine J. Harrison, Helena Kempski, David W. Hammond, Lyndal Kearney 123

10 Fluorescent IgH Fingerprinting to Assess Minimal Residual Disease in Childhood B-Lineage ALL / Paul A. S. Evans, Roger G. Owen 139

11 Real-Time Quantitative RT-PCR to Detect Fusion Gene Transcripts Associated With AML / Rajinder Flora, David Grimwade 151

12 Real-Time PCR to Detect Minimal Residual Disease in Childhood ALL / Cornelia Eckert, Olfert Landt 175

13 Oligonucleotide Microarray Analysis of Gene Expression in Leukemia / Frederik W. van Delft, Louise K. Jones 183

Part VI. Bone Marrow Transplantation

14 HLA Low-Resolution Genotyping for Hematopoietic Stem Cell Transplantation / John Harvey, Jenny Price, Ahna Stanton 199

15 DNA Sequencing as a Tissue-Typing Tool / Paul P. J. Dunn, Sarinder Day, Siaron Williams, Nina Bendukidze 233

16 Analysis of Chimerism After Stem Cell Transplantation / Peter Bader, Hermann Kreyenberg 247.

Notes:

Includes bibliographical references and index.

ISBN:

1588290433

OCLC:

51811269