Gene biotechnology / William Wu ... [and others].

Format:

Book

Contributor:

Wu, William.

Rosengarten Family Fund.

Language:

English

Subjects (All):

Genetic engineering--Laboratory manuals.

Genetic engineering.

Molecular biology--Laboratory manuals.

Molecular biology.

Genre:

Laboratory manuals.

Physical Description:

523 pages : illustrations ; 25 cm

Edition:

Second edition.

Place of Publication:

Boca Raton, Fla. : CRC Press, [2004]

Summary:

Many scientists find themselves working in the laboratory without sufficient background in current biotechnological methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. Now, the information to help you understand and apply today's biological techniques can be found in a single source. Gene Biotechnology, Second Edition provides a multidisciplinary approach to the methods essential to biotechnological development. Written by a team of internationally recognized scientists, the second edition addresses the needs of researchers in academic and commercial environments and serves as a guide for graduate students designing, implementing, and evaluating experimental projects. Presenting many important new methodologies, this unique resource provides you with the tools for success in molecular and cellular biology research today.

Contents:

Chapter 1 Strategies for Novel Research Projects 1

Chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCR 15

Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries 31

Chapter 4 Subcloning of Genes or DNA Fragments 73

Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing 99

Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins 139

Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization 165

Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems 193

Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies 223

Chapter 10 Analysis of Gene Expression at the Functional Genomic Level 247

Chapter 11 Analysis of Gene Expression at the Proteomic Level 265

Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH) 287

Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH) 311

Chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs 323

Chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries 341

Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression 365

Chapter 17 New Strategies for Gene Knockout 375

Chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells 409

Chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR 445

Chapter 20 High-Throughput Analysis of Gene Expression by Cutting-Edge Technology

DNA Microarrays (Gene Chips) 461

Chapter 21 Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology

Phage Display 471

Chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology 493.

Notes:

First ed. published under title: Methods in gene biotechnology. Boca Raton : CRC Press, 1997.

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Rosengarten Family Fund.

ISBN:

0849312884

OCLC:

52288795