RNAi : a guide to gene silencing / edited by Gregory J. Hannon.

Format:

Book

Contributor:

Hannon, Gregory J., 1964-

Hazel M. Hussong Fund.

Language:

English

Subjects (All):

Gene silencing.

RNA.

Gene Silencing.

Medical Subjects:

Gene Silencing.

RNA.

Physical Description:

vii, 436 pages : illustrations (some color) ; 29 cm

Other Title:

RNA interference

Place of Publication:

Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, [2003]

Summary:

In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.

Contents:

1 Sense Cosuppression in Plants: Past, Present, and Future / Richard A. Jorgensen 5

2 Transgene Cosuppression in Animals / James A. Birchler, Manika Pal-Bhadra, Utpal Bhadra 23

3 Regulation of the Genome by Double-stranded RNA / Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, Antonius J.M. Matzke 43

4 RNAi in Caenorhabditis elegans / Rene F. Ketting, Marcel Tijsterman, Ronald H.A. Plasterk 65

5 Small RNAs, Big Biology: Biochemical Studies of RNA Interference / Gregory J. Hannon, Phillip D. Zamore 87

6 shRNA-mediated Silencing of Mammalian Gene Expression / Michael T. McManus, Douglas S. Conklin 109

7 Transposable Elements, RNA Interference, and the Origin of Heterochromatin / Rob Martienssen, Tom Volpe, Zach Lippman, Ann-Valerie Gendrel, Catherine Kidner, Pablo Rabinowicz, Vincent Colot 129

8 The Ribonuclease III Superfamily: Forms and Functions in RNA, Maturation, Decay, and Gene Silencing / Allen W. Nicholson 149

9 RNA-dependent RNA Polymerase in Gene Silencing / Luyun Huang, John Gledhill, Craig E. Cameron 175

10 Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization / Ira M. Hall, Shiv I.S. Grewal 205

11 Total Interference: Genome-wide RNAi Screens in C. elegans / Andrew G. Fraser, Julie Ahringer 233

12 PTGS Approaches to Large-scale Functional Genomics in Plants / Tessa M. Burch-Smith, Jennifer L. Miller, Savithramma P. Dinesh-Kumar 243

13 Mammalian RNA Interference / Thomas Tuschl 265

14 RNAi in Avian Embryos / Esther T. Stoeckli 297

15 Guide to RNAi in Mouse Oocytes and Preimplantation Embryos / Paula Stein, Petr Svoboda 313

16 RNAi Technologies in Drosophila Cell Culture / Scott M. Hammond 345

17 RNAi Applications in Drosophila melanogaster / Richard W. Carthew 361

18 RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms / Elisabetta Ullu, Christian Tschudi 401.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Hazel M. Hussong Fund.

ISBN:

0879696419

0879697040

OCLC:

52216296