Immunohistopathology : a practical approach to diagnosis / Jules M. Elias.

Format:

Book

Author/Creator:

Elias, Jules M.

Language:

English

Subjects (All):

Diagnostic immunohistochemistry--Technique.

Diagnostic immunohistochemistry.

Immunohistochemistry--methods.

Medical Subjects:

Immunohistochemistry--methods.

Physical Description:

xvi, 589 pages : illustrations ; 24 cm

Edition:

Second edition.

Place of Publication:

Chicago : ASCP Press, [2003]

Contents:

1 Immunohistochemical Methods 1

Role of Immunohistochemistry in Diagnostic Pathology 3

Specimen Size and Formaldehyde Fixation 4

Processing and Pretreatment 14

Principles of Immunohistochemical Techniques 29

Tyramide Signal Amplification Technique (TSA) 40

Polyclonal or Monoclonal Antibodies 51

Intensification of Staining by Repeated Application of Reagents 76

Immunostaining of Previously Stained Sections 79

Storage of Antiserum 80

Penetration of Immunoreagents 80

Concentration of Immunologic Reagents 82

Choosing an Immunohistochemical Technique 83

2 Immunohistochemical Identification of the Undifferentiated Tumor and Tumor of Unknown Origin 111

The Diagnostic Approach 111

Algorithmic Interpretation and the Panel Approach 113

Anaplastic Round Cell Tumors in Adults 114

Small Round Blue Cell Tumors of Childhood 118

Fibroblastic Tumors 126

Solitary Fibrous Tumor 128

So-Called "Fibrohistiocytic Tumors" 128

Tumors of Unknown Origin 133

3 The Renal Biopsy 142

Techniques for the Study of Renal Tissue 143

Indications and Contraindications 144

Collection and Transportation 147

Fixation 151

Tissue Processing for Light Microscopy 153

Tissue Processing for Electron Microscopy 154

Cryotomy 154

Sectioning the Paraffin Block 155

Sectioning the Plastic Block 155

Staining for Light Microscopy Morphological Studies 156

Staining of Frozen Sections 159

Staining of Paraffin and Plastic Sections 161

Renal Biopsy in Transplantation 168

Interpretation of Immunofluorescence 169

Immunofluorescence Staining Patterns 170

Complement and Coagulation Components 173

Immune Complex Solubility, Size, Charge, and Avidity 176

Theories of Renal Disease 178

4 The Skin Biopsy 186

Specimen Handling 187

Comparative Advantages of Immunofluorescence and Immunoperoxidase Techniques 190

Staining Procedures 191

Studies in Bullous Disorders 194

Diagnosis of Malignant Melanoma (MM) in Paraffin Sections 213

5 The Lymph Node Biopsy 223

Immunophenotype 224

Distinction of Lymphoid Neoplasms 229

Tissue Preparation 230

Specimen Handling 231

Fine Needle Aspiration Biopsy 234

Transport to Reference Laboratories 234

Frozen Section Fixation 235

Sectioning of Frozen Blocks 237

Paraffin Section Fixation 238

Decalcification 243

Processing and Embedding 244

Cytochemical Staining 245

Enzyme Histochemistry of Frozen, Paraffin, and Plastic Sections 247

Immunostaining of Frozen Sections 248

Immunostaining of Paraffin Sections 258

Malignant Histiocytic Lymphoma 263

Hodgkin's Disease 264

Use of IHC for Dfferential Diagnosis of Large Cell Lymphomas 266

Genotypic Analysis 269

Work-up of a Possible Lymphoma Case 271

6 Morphology, Cytochemistry, and Immunophenotyping of the Acute Leukemias 293

Approaches to Classification of Acute Leukemia 295

Morphological and Cytochemical Features of Lymphoid Leukemias 297

Correlation of Prognosis With FAB Classification 298

Specimen Handling 298

Surface Marker Methodology 299

Immunophenotyping of Leukemic Lymphoblasts 304

Immunologic Analysis of B Lineage ALL 310

Immunologic Analysis of T Lineage ALL 314

Cytochemical and Surface Marker Analysis in Acute Myeloblastic Leukemia 316

Acute Undifferentiated Leukemia 320

Hybrid Leukemia/Biphenotypic Leukemia 320

Chronic Myeloid Leukemia-Blast Crisis 322

Advances in Diagnosing Acute Leukemia 322

7 Sex Steroid Receptors and Biological Markers in Hormone-Dependent Tumors 339

Risk Factors and Prognosis in Breast Cancer 339

Prognostic Value of Hormone Receptors in Breast Carcinoma 340

Antiestrogen Therapeutic Modes 343

Anatomic Localization of ER and PgR 343

Steroid Receptor Detection Methods 345

Quantitation of Assays 352

Optimizing Measurement of Hormone Receptors 357

Enzyme Digestion 366

ER-IHC Quality Assurance 368

Reliability of Postmastectomy ER/PgR Measurement With DCC Assay 371

False-Positive and False-Negative Staining of Paraffin Sections 371

Fine Needle Aspiration (FNA) for ER/PgR Measurement 372

Imprint Smears 376

Steroid Receptors in Uterus and Endometrium Tumors 377

Steroid Receptors in Ovarian Tumors 378

Steroid Receptors in Prostate Cancer 378

ER and PgR: Summary and Future Developments 379

Prognostic Value of Other Proteins in Breast Cancer 380

8 Non-Radioactive In Situ Hybridization for Viral Detection 406

Comparison of Tinctorial Stains, Immunohistochemistry, and In Situ Hybridization 407

Preparation of Target DNA 424

Denaturation or Melting 431

Controls 436

Detection of Hybridized Probe 437

NISH Detection Choices 439

Fluorescence In Situ Hybridization (FISH) 442

Polymerase Chain Reaction (PCR) 443

9 Histochemical and Immunohistochemical Analysis of Amyloid 454

Histochemical Classification 455

Biochemical and Immunohistochemical Classification 456

Histochemical Staining of Amyloid 457

Pretreatment Steps for Histochemical Stains 461

Enzyme Digestion 463

Effects of Fixation 463

Autoclaving 464

Alkaline Guanidine Method 464

Silver Impregnation Techniques 465

Immunohistochemical Staining 465

Immunohistochemical Fixation 467

Types of Amyloid Fibril Proteins 468

Hereditary Cerebral Hemorrhage (Dutch and Icelandic Types) 478

Familial Amyloid Polyneuropathy 479

Long-Term Hemodialysis-Associated Amyloidosis 479

Cutaneous Amyloidosis 480

Amyloid P Component 481

Biopsy Techniques 482

Fat Aspiration vs Tissue Biopsy Techniques 482

Therapy for Amyloidosis 484

Combined Approach for Classifying Amyloids 485

10 Mechanics of Fluorescence Microscopy 500

Light and Color 501

Primary and Secondary Fluorescence 502

Image Intensity and Image Contrast 503

Wavelengths 504

Filter Selection 506

Illumination Incident Light Fluorescence Microscopy 509

Selection of Objectives 512

Fluorescence Fading 513

Fluorescence Photography 513

Fixation and Immunofluorescence Applications 514

Appendix I Heat-Induced Epitope Retrieval Protocols 520

Appendix II Keratin Expression 536

Appendix III Cytokeratin Analysis 561.

Notes:

Includes bibliographical references and index.

ISBN:

0891895027

OCLC:

52505871