Protein bioseparation using ultrafiltration : theory, applications and new developments / Raja Ghosh.

Format:

Book

Author/Creator:

Ghosh, Raja.

Contributor:

J. Fithian Tatem Memorial Fund.

Language:

English

Subjects (All):

Proteins--Separation.

Proteins.

Proteins--Biotechnology.

Ultrafiltration.

Physical Description:

xx, 166 pages : illustrations ; 24 cm

Place of Publication:

London : Imperial College Press ; River Edge, N.J. : Distributed by World Scientific, [2003]

Summary:

Ultrafiltration is a pressure-driven, membrane-based separation process, which is used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications account for nearly 40% of the current total usage of ultrafiltration membranes. Protein bioseparation is an important component of this application segment. Ultrafiltration is used for protein concentration, desalting, clarification and fractionation (i.e. proteinprotein separation). Concentration, desalting and clarification are technologically less demanding and have been in use in the bioprocess industry for some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This book focuses primarily on protein fractionation.

Contents:

Chapter 1 Protein Bioseparation: An Overview 1

1.3 Protein products 2

1.4 The requirement for protein bioseparation 3

1.5 Economic aspects of protein bioseparation 5

1.6 Protein bioseparation methods 5

Chapter 2 Ultrafiltration: An Overview 13

2.3 Advantages of ultrafiltration 15

Chapter 3 Membranes 19

3.2 Membrane material and chemistry 19

3.3 Membrane structure and morphology 21

3.4 Membrane preparation 23

3.5 Driving force in membrane separation processes 24

3.6 Membrane characterisation 26

Chapter 4 Membrane Module and Operation 33

4.1 Membrane elements and modules 33

4.2 Mode of operation 44

Chapter 5 Membrane Fouling 47

5.2 Fouling mechanisms 48

5.3 Chemistry of adsorption 51

5.4 Initial and long-term fouling 52

5.5 Effect of concentration polarisation on fouling 54

5.6 Effect of permeate flux on fouling, and the critical flux concept 54

5.7 Effect of physicochemical parameters on fouling 54

Chapter 6 Permeate Flux in Ultrafiltration 57

6.2 Enhancement of permeate flux 63

6.3 Fouling control 68

Chapter 7 Protein Transmission Through Ultrafiltration Membranes 75

7.1 Protein size 75

Chapter 8 Selectivity of Protein Fractionation in Ultrafiltration 87

8.2 Enhancement of selectivity 89

Chapter 9 Protein Concentration 91

9.2 Solute retention 92

9.3 Permeate flux and fouling in concentration processes 94

9.4 Modules 95

9.5 Mode of operation 96

9.6 Applications 101

Chapter 10 Diafiltration of Protein Solutions 105

10.2 Permeate flux and fouling in diafiltration 105

10.3 Modules 107

10.4 Mode of operation 108

10.5 Applications 108

Chapter 11 Protein Clarification 111

11.2 Protein transmission 111

11.3 Permeate flux and fouling in protein clarification 112

11.4 Particle transmission through UF membrane 112

11.5 Modules 113

11.6 Mode of operation 114

11.7 Applications 118

Chapter 12 Protein Fractionation 121

12.2 Challenges facing ultrafiltration based protein fractionation 121

12.3 Selectivity enhancement 123

12.4 Thumb rules for protein fractionation 133

12.5 Application areas 135

Chapter 13 New Developments 145

13.1 New membranes 145

13.2 New membrane modules 146

13.3 Rapid process optimisation techniques 147

13.4 New modes of operation 148.

Notes:

Includes bibliographical references.

Local Notes:

Acquired for the Penn Libraries with assistance from the J. Fithian Tatem Memorial Fund.

ISBN:

1860943179

OCLC:

52901015