PCR protocols / edited by John M.S. Bartlett and David Stirling.

Format:

Book

Contributor:

Bartlett, John M. S.

Stirling, David.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 226.

Methods in molecular biology, 1064-3745 ; v. 226

Language:

English

Subjects (All):

Polymerase chain reaction--Laboratory manuals.

Polymerase chain reaction.

Polymerase Chain Reaction.

Medical Subjects:

Polymerase Chain Reaction.

Genre:

Laboratory manuals.

Physical Description:

xvii, 545 pages : illustrations ; 26 cm.

Edition:

Second edition.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Notes:

Includes bibliographical references and index.

ISBN:

0896036421

0896036278

OCLC:

51031066