Membrane protein protocols : expression, purification, and characterization / edited by Barry S. Selinsky.

Format:

Book

Contributor:

Selinsky, Barry Steven.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 228.

Methods in molecular biology ; v.228

Language:

English

Subjects (All):

Membrane proteins--Laboratory manuals.

Membrane proteins.

Membrane Proteins.

Medical Subjects:

Membrane Proteins.

Genre:

Laboratory manuals.

Physical Description:

xiv, 334 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.

Contents:

Part I. Expression of Membrane Proteins

1 Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b[subscript 5] in Escherichia coli / Lucy Waskell 3

2 Dihydroorotate Dehydrogenase of Escherichia coli / Kaj Frank Jensen, Sine Larsen 11

3 A General Approach for Heterologous Membrane Protein Expression in Escherichia coli: The Uncoupling Protein, UCP1, as an Example / Alison Z. Shaw, Bruno Miroux 23

4 Expression of Membrane-Bound Iron-Sulfur Proteins / Dirk Schneider, Christian L. Schmidt, Andreas Seidler 37

5 Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris / Chang-Hoon Han, Moritz Werder, Wilk von Gustedt, Helmut Hauser 49

6 Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells / U. Subrahmanyeswara Rao 65

7 Functional Expression of His-Tagged Rhodopsin Sf9 Insect Cells / Giel J.C.G.M. Bosman, Jenny van Oostrum, Githa Breikers, Petra H.M. Bovee-Geurts, Corne H.W. Klaassen, Willem J. DeGrip 73

Part II. Detergent Selection in Membrane Protein Purification

8 Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution / Darren Manley, Joe D. O'Neil 89

9 Solubilization of Chemokine Receptors from Cell Membranes / Robert Staudinger, Juan C. Bandres 103

10 A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid: Acyl-CoA Acyltransferase (LAT) / Claus Kerkhoff, Volkhard Kaever 111

Part III. Membrane Protein Purification and Reconstitution

11 Purification of Omp50, a Minor Porin of Campylobacter jejuni / Jean Michel Bolla 131

12 Purification of Porins from Mycobacterium smegmatis / Christian Heinz, Eva Roth, Michael Niederweis 139

13 Isolation of the Melanocortin 5 Receptor: From cDNA Sequence to Isolating an Integral Membrane Protein / Benjamin L. Clarke 151

14 Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography / Kentaro Hanada, Masahiro Nishijima 163

15 Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells / Kiyoshi Kawasaki, Masahiro Nishijima 175

16 Purification of Pancreastatin Receptor from Rat Liver Membranes / Victor Sanchez-Margalet, Jose Santos-Alvarez, Sandra Diaz-Troya 187

17 Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes / Rajendra Prasad 195

18 Isolation of Lipid Raft-Associated Proteolipids / Jaime Millan, Maria C. de Marco, Mohammed Qaidi, Alicia Batista, Fernando Martin-Belmonte, Miguel A. Alonso 223

19 Purification of Membrane-Bound Catechol-O-Methyltransferase / Maria Joao Bonifacio, Patricio Soares-da-Silva 231

20 Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane / Da-Neng Wang, M. Joanne Lemieux, Jonathan M. Boulter 239

21 Purification of the Human Erythrocyte PS-Stimulated Mg[superscript 2+]-ATPase: A Putative PS Flippase / Jill V. Lyles, Kathleen Cornely-Moss, Christine M. Smith, David L. Daleke 257

22 Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes / Sathyanarayana N. Gummadi, Sigrun Hrafnsdottir, Jane Walent, William E. Watkins, Anant K. Menon 271

23 Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I / Nicholas F. Brown 281

Part IV. Structural Analysis of Membrane Proteins

24 Crystallization in Lipidic Cubic Phases: A Case Study with Bacteriorhodopsin / Valentin I. Gordeliy, Ramona Schlesinger, Rouslan Efremov, Georg Buldt, Joachim Heberle 305

25 Optical Biosensor Assay Using Retroviral Receptor Pseudotypes / Joseph Rucker 317.

Notes:

Includes bibliographical references and index.

ISBN:

1588291243

OCLC:

51817240