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p53 protocols / edited by Sumitra Deb and Swati Palit Deb.
Holman Biotech Commons QH506 .M45 v.234 2003
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; v. 234.
- Methods in molecular biology ; v. 234
- Language:
- English
- Subjects (All):
- p53 protein--Laboratory manuals.
- p53 protein.
- p53 antioncogene--Laboratory manuals.
- p53 antioncogene.
- Protein p53--analysis.
- Protein p53--therapeutic use.
- Tumor Suppressor Proteins--analysis.
- Genes, p53--physiology.
- Neoplasms--genetics.
- Medical Subjects:
- Protein p53--analysis.
- Protein p53--therapeutic use.
- Tumor Suppressor Proteins--analysis.
- Genes, p53--physiology.
- Neoplasms--genetics.
- Genre:
- Laboratory manuals.
- Physical Description:
- xi, 280 pages : illustrations ; 24 cm.
- Place of Publication:
- Totowa, N.J. : Humana Press, 2003.
- Summary:
- Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. Described by leading basic and clinical researchers who have successfully used the methods, the techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. Special effort has been made to discuss problems and their solutions to help ease the learning curve when standardizing a new method. Diverse and highly practical, p53 Protocols offers both beginning and experienced researchers in cancer biology a gold-standard compendium of readily reproducible techniques for studying p53 and related proteins.
- Contents:
- 1 Adenovirus Expressing p53 / Matthew Holmes, Elizabeth Rosenberg, Kristoffer Valerie 1
- 2 Purification of Recombinant p53 from Sf9 Insect Cells / Xiu Zhu Sun, John Nguyen, Jamil Momand 17
- 3 Generation and Characterization of p53 Mutant Mice / Melissa L. Dumble, Lawrence A. Donehower, Xiongbin Lu 29
- 4 Identification of p53 Target Genes by Fluorescent Differential Display / Susanne Stein, Yong-Jig Cho, Roger S. Jackson II, Peng Liang 51
- 5 p53-Induced Gene Expression Analysis / Joanna K. Sax, Wafik S. El-Deiry 65
- 6 Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR Green I / Katherine E. Roth Stagliano, Evie Carchman, Sumitra Deb 73
- 7 Transactivation and Transrepression Studies with p53 / Mariano J. Scian, Rebecca Frum, Swati Palit Deb, Sumitra Deb 93
- 8 Methods to Study p53-Represented Promoters / Patrick Dumont, Anthony Della Pietra, Maureen E. Murphy 111
- 9 Interaction of p53 with Cellular Proteins / David M. Keller, Shelya X. Zeng, Hua Lu 121
- 10 Interaction of p53 with the Adenovirus E1B-55 kDa Protein / Judith Roth, Matthias Dobbelstein 135
- 11 In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability / Maria Patricia Molina, Christine Cain, Jill Bargonetti 151
- 12 Signaling to p53: The Use of Phospho-Specific Antibodies to Probe for In Vivo Kinase Activation / Ashley L. Craig, Susan E. Bray, Lee E. Finlan, Neil M. Kernohan, Ted R. Hupp 171
- 13 Detection of Mitochondrial Localization of p53 / Motohiro Mihara, Ute M. Moll 203
- 14 Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress / Troy W. Joseph, Ute M. Moll 211
- 15 Mutational Analysis of p53 in Human Tumors: Direct DNA Sequencing and SSCP / Susan Erster, Neda Slade, Ute M. Moll 219
- 16 Mutational Analysis of p53 in Human Tumors: Immunocytochemistry / Neda Slade, Ute M. Moll 231
- 17 Flow Cytometric Analysis of p53-Induced Apoptosis / Michael Berger, Ygal Haupt 245
- 18 Flow Cytometric Analysis of MDM2-Mediated Growth Arrest / Rebecca Frum, Swati Palit Deb 257.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 1588291065
- OCLC:
- 51171849
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