Protein kinase C protocols / edited by Alexandra C. Newton.

Format:

Book

Contributor:

Newton, Alexandra C.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 233.

Methods in molecular biology ; 233

Language:

English

Subjects (All):

Protein kinase C--Laboratory manuals.

Protein kinase C.

Protein Kinase C.

Signal Transduction.

Medical Subjects:

Protein Kinase C.

Signal Transduction.

Genre:

Laboratory manuals.

Physical Description:

xvi, 582 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

The first compilation of classic and emerging laboratory techniques for the study of the biochemistry and cell biology of protein kinase C (PKC). Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C.. Each protocol is introduced in the context of PKC function and regulation and contains many notes on how best to deal with the problems that may occur. Comprehensive and authoritative, Protein Kinase C Protocols is a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that brings protein kinase C research into any laboratory interested in studying it.

Contents:

1 The Ins and Outs of Protein Kinase C / Alexandra C. Newton 3

2 Early Studies of Protein Kinase C: A Historical Perspective / Yasutomi Nishizuka, Ushio Kikkawa 9

Part II. Expression and Purification

3 Expression and Purification of Protein Kinase C from Insect Cells / Hideyuki Mukai, Yoshitaka Ono 21

4 Expression and Purification of Protein Kinase C[delta] from Bacteria / Michael Gschwendt, Luise Stempka 35

Part III. Measuring the Activity of Protein Kinase C

5 Complexities in Protein Kinase C Activity Assays: An Introduction / Julianne J. Sando 45

6 Enzyme Assays for Protein Kinase C Activity / Julianne J. Sando, Jacquelyn K. Beals 63

7 Subcellular Translocation of Protein Kinase C / Julianne J. Sando, Jacquelyn K. Beals, Isa M. Hussaini 77

Part IV. Measuring the Interaction of Protein Kinase C with Membranes

8 Measuring the Interaction of Protein Kinase C with Membranes: An Introduction / Alexandra C. Newton 89

9 Fluorescence Imaging of Protein Kinase C Translocation in Living Cells / Naoaki Saito 93

10 Measuring the Binding of Protein Kinase C to Sucrose-Loaded Vesicles / Jennifer Giorgione, Alexandra C. Newton 105

11 Use of Stopped-Flow Fluorescence Spectroscopy to Measure Rapid Membrane Binding by Protein Kinase C / Eric A. Nalefski, Alexandra C. Newton 115

12 [superscript 3 H]Phorbol 12, 13-Dibutyrate Binding Assay for Protein Kinase C and Related Proteins / Nancy E. Lewin, Peter M. Blumberg 129

Part V. Measuring the Phosphorylation of Protein Kinase C

13 Protein Kinase C Phosphorylation: An Introduction / Peter J. Parker 159

14 Pulse-Chase Analysis of Protein Kinase C / Mikiko Takahashi, Yoshitaka Ono 163

15 PDK-1 and Protein Kinase C Phosphorylation / Alex Toker 171

16 In Vitro Autophosphorylation of Protein Kinase C Isozymes / Antonio M. Pepio, Wayne S. Sossin 191

17 Tyrosine Phosphorylation of Protein Kinase C / Toshiyoshi Yamamoto, Emiko Yamauchi, Hisaaki Taniguchi, Hidenori Matsuzaki, Ushio Kikkawa 207

18 Methods to Study Dephosphorylation of Protein Kinase C In Vivo / Karen England, Martin Rumsby 217

19 Phosphopeptide-Specific Antibodies to Protein Kinase C / Wayne S. Sossin 233

Part VI. Identifying Protein Kinase C Substrates

20 Identifying Protein Kinase C Substrates: An Introduction / Alex Toker 247

21 A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases / Kavita Shah, Kevan M. Shokat 253

22 Studying the Optimal Peptide Substrate Motifs of Protein Kinase C Using Oriented Peptide Libraries / Michael B. Yaffe 273

Part VII. Structural Analysis of Protein Kinase C

23 Structural Analysis of Protein Kinase C: An Introduction / James H. Hurley 289

24 Bacterial Expression and Purification of C1 and C2 Domains of Protein Kinase C Isoforms / Wonhwa Cho, Michelle Digman, Bharath Ananthanarayanan, Robert V. Stahelin 291

25 Crystallization of the Protein Kinase C[delta] C1B Domain / Gongyi Zhang, James H. Hurley 299

Part VIII. Methods for Detecting Binding Proteins

26 Methods for Detecting Binding Proteins: An Introduction / Gerda Endemann, Daria Mochly-Rosen 307

27 Detection of Protein Kinase-Binding Partners by the Yeast Two-Hybrid Analysis / Betty Y. Chang, Christine A. Cartwright 327

28 Glutathione S-Transferase Pull-Down Assay / Deborah Schechtman, Daria Mochly-Rosen, Dorit Ron 345

29 Overlay Method for Detecting Protein-Protein Interactions / Deborah Schechtman, Christopher Murriel, Rachel Bright, Daria Mochly-Rosen 351

30 An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates / Susan Jaken 359

31 Functional Proteomic Analysis of the Protein Kinase C Signaling System / Jason M. Pass, Jun Zhang, Thomas M. Vondriska, Peipei Ping 369

Part IX. Pharmacological Probes for Protein Kinase C

32 Pharmacological Probes for Protein Kinase C: An Introduction / Marcelo G. Kazanietz 389

33 Applications of Inhibitors for Protein Kinase C and Their Isoforms / Garry X. Shen, Kerrie J. Way, Judith R. C. Jacobs, George L. King 397

34 Phorbol Esters as Probes for the Study of Protein Kinase C Function / Marcelo G. Kazanietz, Patricia S. Lorenzo 423

35 Irreversible Inactivation of Protein Kinase C Isozymes by Thiol-Reactive Peptide Substrate Analogs / Catherine A. O'Brian, Nancy E. Ward, Feng Chu, Jubilee R. Stewart 441

Part X. Genetic Approaches to Studying Protein Kinase C

36 Genetic Approaches to Studying Protein Kinase C: An Introduction / Robert O. Messing 453

37 Animal Models in the Study of Protein Kinase C Isozymes / Doo-Sup Choi, Robert O. Messing 455

38 Genetic Manipulation of Protein Kinase C In Vivo / Alex Toker 475

39 Yeast as a Host to Screen for Modulators and Regulatory Regions of Mammalian Protein Kinase C Isoforms / Amadeo M. Parissenti, Heimo Riedel 491

Part XI. Protein Kinase C in Disease

40 Protein Kinase C in Disease: Cancer / Alan P. Fields, W. Clay Gustafson 519

41 Characterization of the Role of Protein Kinase C Isozymes in Colon Carcinogenesis Using Transgenic Mouse Models / Alan P. Fields, Nicole R. Murray, W. Clay Gustafson 539

42 Alcohol Addiction / M. Foster Olive, Dorit Ron 555.

Notes:

Includes bibliographical references and index.

ISBN:

1588290689

OCLC:

51817366