Membrane transporters : methods and protocols / edited by Qing Yan.

Format:

Book

Contributor:

Yan, Qing, 1971-

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 227.

Methods in molecular biology ; v. 227

Language:

English

Subjects (All):

Carrier proteins--Laboratory manuals.

Carrier proteins.

Membrane proteins--Laboratory manuals.

Membrane proteins.

Physiological transport, Active--Laboratory manuals.

Physiological transport, Active.

Ion channels--Laboratory manuals.

Ion channels.

Membrane Transport Proteins.

Pharmacogenetics.

Medical Subjects:

Membrane Transport Proteins.

Pharmacogenetics.

Genre:

Laboratory manuals.

Physical Description:

xii, 369 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

Membrane transporters and channels play key roles in drug absorption and distribution, influence drug efficacy and toxicity, and are the target of about 30% of currently marketed drugs. In Membrane Transporters: Methods and Protocols, a panel of recognized basic and clinical investigators -- many world-famous -- describe their proven, cutting-edge methodologies for studying these important proteins. Treated in step-by-step detail, these techniques take advantage of all the latest developments in biomedical research, including pharmacogenomics, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics analysis, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small-angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microscopy, are also described, along with several methods that may help in the development of novel therapeutics. Each technique contains hands-on instructions, many notes on avoiding the problems that one may encounter, and suggestions for variant procedures. Comprehensive and highly practical, Membrane Transporters: Methods and Protocols offers investigators working in biopharmaceutical science, drug discovery, and drug delivery both an array of powerful tools for work on membrane transporters and an essential guide to the state-of-the-art in the membrane transporter field today.

Contents:

1 Pharmacogenomics of Membrane Transporters: An Overview / Qing Yan 1

2 The IUBMB-Endorsed Transporter Classification System / Wolfgang Busch, Milton H. Saier, Jr. 21

3 Bioinformatics and Data Integration in Membrane Transporter Studies / Qing Yan 37

4 DNA Microarrays to Analyze Gene Expression in Animals With Altered Transporter Expression / Gary W. Miller 61

5 Microarray Data Analysis in Studies of Membrane Transporters / Donglei Hu 71

6 Laser Capture Microdissection to Examine Transporter Expression in Specific Cell Regions / Okkyung Rho, Gary W. Miller 85

7 Site-Directed Mutagenesis in the Study of Membrane Transporters / Renae M. Ryan, Ann D. Mitrovic, Robert J. Vandenberg 97

8 Fluorescence Techniques for Studying Membrane Transport Proteins: The P-Glycoprotein Multidrug Transporter / Frances J. Sharom, Paula L. Russell, Qin Qu, Peihua Lu 109

9 Reconstitution of Membrane Transporters / Frances J. Sharom, Paul D. W. Eckford 129

10 Equilibrium Binding and Transport Studies / Ana M. Ojeda, Dawn T. Bravo, Terry L. Hart, Stanley M. Parsons 155

11 Electrophysiological Studies of lon Channels and Therapeutic Potential With Adenovirus-Mediated Gene Transfer / Jihong Qu, Thai V. Pham, Maria N. Obreztchikova 179

12 Antibody Production and Immunocytochemical Localization of Amino Acid Transporters / David V. Pow, Robert Sullivan, Heather Scott 213

13 Xenopus laevis Oocytes / Stefan Broer 245

14 Measurement of Intracellular pH / Frederick B. Loiselle, Joseph R. Casey 259

15 Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domains / Brian H. Shilton 281

16 Membrane Protein NMR Studies / Stanley J. Opella 307

17 Analysis of Soluble Sugar Permease Domains by Solution NMR / Thomas A. Wilkinson, Yuan Chen 321

18 Molecular Modeling and Molecular Dynamics Simulations of Membrane Transporter Proteins / Donard S. Dwyer 335

19 Imaging Plasma Membrane Proteins by Confocal Microscopy / Stella W. Y. Tsang, Peter M. Haney 351.

Notes:

Includes bibliographical references and index.

ISBN:

1588291049

OCLC:

51022763