Lentivirus gene engineering protocols / edited by Maurizio Federico.

Format:

Book

Contributor:

Federico, Maurizio.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 229.

Methods in molecular biology ; v. 229

Language:

English

Subjects (All):

Genetic engineering.

Lentiviruses.

Genetic vectors.

Lentivirus--genetics.

Genetic Vectors.

Transduction, Genetic-Laboratory Manuals.

Genetic Engineering--methods.

Medical Subjects:

Lentivirus--genetics.

Genetic Vectors.

Transduction, Genetic-Laboratory Manuals.

Genetic Engineering--methods.

Genre:

Laboratory manuals.

Physical Description:

xi, 314 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2003.

Summary:

Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.

Contents:

Part I. Lentivirus Vectors

1 From Lentiviruses to Lentivirus Vectors / Maurizio Federico 3

2 The Choice of a Suitable Lentivirus Vector: Transcriptional Targeting / Francesco Lotti, Fulvio Mavilio 17

3 Choice and Use of Appropriate Packaging Cell Types / Annmarie L. Pacchia, Sayandip Mukherjee, Joseph P. Dougherty 29

4 Small- to Large-Scale Production of Lentivirus Vectors / Michael P. Marino, Milson J. Luce, Jakob Reiser 43

5 Detection and Titration of Lentivirus Vector Preparations / Joel Gatlin, Miguel Islas-Ohlmayer, J. Victor Garcia 57

6 Detection and Selection of Lentiviral Vector-Transduced Cells / Yan Cui, Lung-Ji Chang 69

7 Detection of Replication-Competent Lentiviral Particles / Harry Segall, Richard E. Sutton 87

Part II. Transduction of Ex Vivo Cell Cultures Through Lentivirus Vectors

8 Lymphocytes / Dhanalakashmi Chinnasamy, Fabio Candotti 97

9 Monocyte/Macrophages and Dendritic Cells / Harry Segall, Richard E. Sutton 107

10 Hematopoietic Stem and Progenitor Cells / Robert E. Donahue, Irvin S. Y. Chen 117

11 Mesenchymal Stem Cells / Xian-Yang Zhang, Vincent F. La Russa, Jakob Reiser 131

12 Hippocampal Neurons / Jay L. Nadeau 141

13 Dorsal Root Ganglia Sensory Neurons / Jane Fleming, Samantha L. Ginn, Ian E. Alexander 155

14 Cardiomyocytes / Desiree Bonci, Michael V. G. Latronico, Gianluigi Condorelli 169

15 Airway Epithelia / Larry G. Johnson, Scott H. Randell, John C. Olsen 181

16 Corneal Cells / Binoy Appukuttan, Trevor McFarland, J. Timothy Stout 197

17 Retinal Tissue / Trevor McFarland, Binoy Appukuttan, J. Timothy Stout 205

Part III. Advantages and Uses of Non-HIV-1-Based Lentivirus Vectors

18 HIV-2 Vectors / Suresh K. Arya, Jean E. Cho, Suzie H. Chang 219

19 SIV Vectors / Alessia Ruggieri, Didier Negre, Francois-Loic Cosset 233

20 FIV Vectors / Nils Loewen, Roman Barraza, Todd Whitwam, Dyana T. Saenz, Iris Kemler, Eric M. Poeschla 251

21 Hybrid Lentivirus Vectors / Vicente Planelles 273

Part IV. Direct In Vivo Transduction by Lentivirus Vector and Detection

22 Cells of Respiratory Epithelium / Patrick L. Sinn, Paul B. McCray, Jr. 287

23 Cells of the Nervous System / Carl Rosenblad, Cecilia Lundberg 299.

Notes:

Includes bibliographical references and index.

ISBN:

1588290913

OCLC:

51046957