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E. coli plasmid vectors : methods and applications / edited by Nicola Casali and Andrew Preston.
Holman Biotech Commons QH506 .M45 v.235 2003
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; v. 235.
- Methods in molecular biology ; v. 235
- Language:
- English
- Subjects (All):
- Plasmids--Laboratory manuals.
- Plasmids.
- Escherichia coli--Laboratory manuals.
- Escherichia coli.
- Genetic vectors--Laboratory manuals.
- Genetic vectors.
- Plasmids--genetics.
- Genetic Vectors.
- Escherichia coli--genetics.
- Cloning, Molecular--methods.
- DNA, Recombinant.
- Medical Subjects:
- Plasmids--genetics.
- Genetic Vectors.
- Escherichia coli--genetics.
- Cloning, Molecular--methods.
- DNA, Recombinant.
- Genre:
- Laboratory manuals.
- Physical Description:
- xi, 316 pages : illustrations ; 24 cm.
- Place of Publication:
- Totowa, N.J. : Humana Press, [2003]
- Summary:
- A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
- Contents:
- 1 The Function and Organization of Plasmids / Finbarr Hayes 1
- 2 Choosing a Cloning Vector / Andrew Preston 19
- 3 Escherichia coli Host Strains / Nicola Casali 27
- 4 Chemical Transformation of E. coli / W. Edward Swords 49
- 5 Electroporation of E. coli / Claire A. Woodall 55
- 6 DNA Transfer by Bacterial Conjugation / Claire A. Woodall 61
- 7 Cosmid Packaging and Infection of E. coli / Mallory J. A. White, Wade A. Nichols 67
- 8 Isolation of Plasmids from E. coli by Alkaline Lysis / Sabine Ehrt, Dirk Schnappinger 75
- 9 Isolation of Plasmids from E. coli by Boiling Lysis / Sabine Ehrt, Dirk Schnappinger 79
- 10 High-Purity Plasmid Isolation Using Silica Oxide / Stefan Grimm, Frank Voss-Neudecker 83
- 11 High-Throughput Plasmid Extraction Using Microtiter Plates / Michael A. Quail 89
- 12 Isolation of Cosmid and BAC DNA from E. coli / Daniel Sinnett, Alexandre Montpetit 99
- 13 Preparation of Single-Stranded DNA from Phagemid Vectors / W. Edward Swords 103
- 14 Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects / Robert H. Gross 107
- 15 Cloning in Plasmid Vectors / Carey Pashley, Sharon Kendall 121
- 16 Extraction of DNA from Agarose Gels / Nicholas Downey 137
- 17 Cloning PCR Products with T-Vectors / Wade A. Nichols 141
- 18 Construction of Genomic Libraries in [gamma]-Vectors / Yilun Wang, Zheng Cao, Darryl Hood, James G. Townsel 153
- 19 Rapid Screening of Recombinant Plasmids / Sangwei Lu 169
- 20 Restriction Analysis of Recombinant Plasmids / Joanne Goranson-Siekierke, Jarrod L. Erbe 175
- 21 Screening Recombinant DNA Libraries / Wade A. Nichols 183
- 22 Sequencing Using Fluorescent-Labeled Nucleotides / Allison F. Gillaspy 195
- 23 Site-Directed Mutagenesis Using the Megaprimer Method / Zhidong Xu, Alessia Colosimo, Dieter C. Gruenert 203
- 24 Site-Directed Mutagenesis by Inverse PCR / Clifford N. Dominy, David W. Andrews 209
- 25 Creating Nested DNA Deletions Using Exonuclease III / Rosamund Powles, Lafras M. Steyn 225
- 26 Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs / Alistair McGregor 233
- 27 In Vitro Transcription and Translation / Farahnaz Movahedzadeh, Susana Gonzalez Rico, Robert A. Cox 247
- 28 Vectors for the Expression of Recombinant Proteins in E. coli / Sally A. Cantrell 257
- 29 Expression of Recombinant Proteins from Iac Promoters / Charles R. Sweet 277
- 30 Plasmid-Based Reporter Genes: Assays for [beta]-Galactosidase and Alkaline Phosphatase Activities / Minghsun Liu 289
- 31 Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein / Sergei R. Doulatov 297.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 1588291510
- OCLC:
- 51305670
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