Cell migration in inflammation and immunity : methods and protocols / edited by Daniel D'Ambrosio and Francesco Sinigaglia.

Format:

Book

Contributor:

D'Ambrosio, Daniele.

Sinigaglia, Francesco.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 239.

Methods in molecular biology, 1064-3745 ; 239

Language:

English

Subjects (All):

Chemokines--Laboratory manuals.

Chemokines.

Chemokines--Receptors--Laboratory manuals.

Leucocytes--Motility--Laboratory manuals.

Leucocytes.

Inflammation--Mediators--Laboratory manuals.

Inflammation.

Chemokines--immunology.

Inflammation--Mediators.

Leucocytes--Motility.

Receptors, Chemokine--immunology.

Cell Movement--immunology.

Leukocytes--immunology.

Immunologic Techniques.

Medical Subjects:

Chemokines--immunology.

Receptors, Chemokine--immunology.

Cell Movement--immunology.

Leukocytes--immunology.

Immunologic Techniques.

Genre:

Laboratory manuals.

Physical Description:

xii, 277 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2004]

Summary:

Cell migration is now well recognized as a critical component of the inflammatory disease process, so that its proper understanding promises to generate both ground-breaking basic discoveries and the development of novel therapeutics. In Cell Migration in Inflammation and Immunity: Methods and Protocols, leading cell biologists and immunologists present their most widely useful and innovative techniques for studying the molecular and cellular basis of this phenomenon. Describing each method in step-by-step detail, the authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying both vascular recognition and leukocyte-endothelium interaction. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and cronic inflammatory diseases are emphasized. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls. Comprehensive and cutting-edge, Cell Migration in Inflammation and Immunity: Methods and Protocols offers novice and experienced investigators alike a collection of powerful techniques for studying the molecular basis and pathophysiological significance of cell migration in inflammatory and immune diseases, as well as for the development of novel therapeutics.

Contents:

1 Chemotaxis and Interaction with Vascular or Lymphatic Endothelium / Silvano Sozzani, Annunciata Vecchi, Paola Allavena, Alberto Mantovani 1

2 Analysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow Conditions / Carlo Laudanna 17

3 Posttranslational Processing of Chemokines / Paul Proost, Frank Mahieu, Evemie Schutyser, Jo Van Damme 27

4 Chemotactic Profiling of Lymphocyte Subpopulations / Lucia Colantonio, Andrea Iellem, Daniele D'Ambrosio 45

5 Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow Cytometry / Miguel Vicente-Manzanares, Mariano Viton, Francisco Sanchez-Madrid 53

6 Evaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation on Human Leukocytes / Angela Gismondi, Fabrizio Mainiero, Angela Santoni 69

7 Reconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted Tracking / Peter Friedl, Eva-B. Brocker 77

8 Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System / Mark A. Landree, Peter N. Devreotes 91

9 Conditional Transgenic Models to Study Chemokine Biology / Sergio A. Lira, Borna Mehrad, Shu-Cheng Chen, Petronio Zalamea, David J. Kinsley, Maria T. Wiekowski, Elizabeth Coronel, Galya Vassileva, Denise Manfra, Kristian K. Jensen 105

10 Intravital Microscopy as a Tool for Studying Recruitment and Chemotaxis / Denise C. Cara, Paul Kubes 123

11 Tracking Antigen-Specific Lymphocytes In Vivo / Claire L. Adams, Catherine M. Rush, Karen M. Smith, Paul Garside 133

12 Analysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease States / Margherita Mariani, Paola Panina-Bordignon 147

13 Interaction of Viral Chemokine Inhibitors with Chemokines / Antonio Alcami 167

14 Discovery of Small-Molecule Antagonists of Chemokine Receptors: Screening Strategy and Assays / Maria Elena Fuentes 181

15 Visualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital Microscopy / Gabriela Constantin 189

16 Animal Models to Study Chemokine Receptor Function In Vivo: Mouse Models of Allergic Airway Inflammation / Clare M. Lloyd, Jose-Carlos Gutierrez-Ramos 199

17 Assessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling: Use of Dominant Negative Mutants Controlled by a Tetracycline-Regulated Gene Expression System / Adam P. Curnock, Yannis Sotsios, Stephen G. Ward 211

18 In Vitro and In Vivo Models to Study Chemokine Regulation of Angiogenesis / Giovanni Bernardini, Domenico Ribatti, Gaia Spinetti, Lucia Morbidelli, Marina Ziche, Angela Santoni, Maurizio C. Capogrossi, Monica Napolitano 223

19 Real Time In Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow Conditions / Guy Cinamon, Ronen Alon 233

20 Generation of Monoclonal Antibodies Against Chemokine Receptors / Leonor Kremer, Gabriel Marquez 243

21 Detection of High-Affinity [alpha]4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines / Jason R. Chan, Myron I. Cybulsky 261.

Notes:

Includes bibliographical references and index.

ISBN:

1588291022

OCLC:

51848414