The blood-brain barrier : biology and research protocols / edited by Sukriti Nag.

Format:

Book

Contributor:

Nag, Sukriti.

Series:

Methods in molecular medicine ; 89.

Methods in molecular medicine ; 89

Language:

English

Subjects (All):

Blood-brain barrier--Laboratory manuals.

Blood-brain barrier.

Blood-Brain Barrier--physiology.

Clinical Protocols.

Molecular Biology--methods.

Molecular Diagnostic Techniques--methods.

Medical Subjects:

Blood-Brain Barrier--physiology.

Clinical Protocols.

Molecular Biology--methods.

Molecular Diagnostic Techniques--methods.

Genre:

Laboratory manuals.

Physical Description:

xiii, 549 pages : illustrations (some color) ; 26 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2003.

Summary:

Despite the fact that the breakdown of the blood-brain barrier occurs in many brain diseases and is an important factor in mortality, the molecular mechanisms involved in the breakdown are not completely understood. In The Blood-Brain Barrier: Biology and Research Protocols, leading researchers and clinician-scientists present state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium. Comprehensive and state-of-the-art, The Blood-Brain Barrier: Biology and Research Protocols offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.

Contents:

Part I Tissue Techniques

1 Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels / Sukriti Nag 3

2 Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry / Sukriti Nag 37

3 Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions / Hartwig Wolburg, Stefan Liebner, Andrea Lippoldt 51

4 Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy / Albert S. Lossinsky, Richard R. Shivers 67

5 Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy / Britta Engelhardt, Peter Vajkoczy, Melanie Laschinger 83

Part II Blood-Brain Barrier Permeability Techniques

6 Pathophysiology of Blood-Brain Barrier Breakdown / Sukriti Nag 97

7 Pial Vessel Permeability to Tracers Using Cranial Windows / William G. Mayhan 121

8 Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry / Sukriti Nag 133

9 Ultracytochemical Studies of the Compromised Blood-Brain Barrier / Sukriti Nag 145

10 Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier / Eain M. Cornford, Shigeyo Hyman, Marcia E. Cornford 161

11 Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [superscript 14 C]-Labeled Tracers / Kamlesh Asotra, Nagendra Ningaraj, Keith L. Black 177

Part III Blood-Brain Barrier Transport Techniques

12 A Review of Blood-Brain Barrier Transport Techniques / Quentin R. Smith 193

13 In Situ Brain Perfusion Technique / Quentin R. Smith, David D. Allen 209

14 Brain Efflux Index Method: Characterization of Efflux Transport Across the Blood-Brain Barrier / Hiroyuki Kusuhara, Tetsuya Terasaki, Yuichi Sugiyama 219

15 Transport Studies Using Membrane Vesicles / Darryl R. Peterson, Richard A. Hawkins 233

16 Drug Transport Studies Using Quantitative Microdialysis / Haiqing Dai, William F. Elmquist 249

17 Single-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic Acids / Michelle A. Puchowicz, Kui Xu, Joseph C. LaManna 265

18 Protein Transport in Cerebral Endothelium: In Vitro Transcytosis of Transferrin / Laurence Fenart, Romeo Cecchelli 277

19 Transport Across the Choroid Plexuses in Vivo and In Vitro / Nathalie Strazielle, Jane Elizabeth Preston 291

Part IV In Vitro Techniques

20 An Overview of In Vitro Techniques for Blood-Brain Barrier Studies / Andreas Reichel, David J. Begley, N. Joan Abbott 307

21 Isolation and Characterization of Human Brain Endothelial Cells / Katerina Dorovini-Zis, Rukmini Prameya, Hanh Huynh 325

22 Human Brain Microvessel Endothelial Cell and Leukocyte Interactions / Jacqueline Shukaliak-Quandt, Donald Wong, Katerina Dorovini-Zis 337

23 Development and Characterization of Immortalized Cerebral Endothelial Cell Lines / Pierre-Olivier Couraud, John Greenwood, Francoise Roux, Pete Adamson 349

24 Isolation and Characterization of Retinal Endothelial Cells / David A. Antonetti, Ellen B. Wolpert 365

25 Isolation and Characterization of Cerebral Microvascular Pericytes / Paula Dore-Duffy 375

Part V Molecular Techniques

26 Molecular Biology of the Blood-Brain Barrier / William M. Pardridge 385

27 Blood-Brain Barrier Genomics / Ruben J. Boado 401

28 Dynamic In Vitro Model of the Blood-Brain Barrier: Gene Profiling Using cDNA Microarray Analysis / Matteo Marroni, Kelly M. Kight, Mohammed Hossain, Luca Cucullo, Shailesh Y. Desai, Damir Janigro 419

29 Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells / Jamshid Tanha, Arumugam Muruganandam, Danica Stanimirovic 435

30 mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization / David Zagzag, Wai Chan 451

31 Proteomics of Brain Endothelium: Separation of Proteins by Two-Dimensional Gel Electrophoresis and Identification by Mass Spectrometry / Reiner F. Haseloff, Eberhard Krause, Ingolf E. Blasig 465

32 Immunoblot Detection of Brain Vascular Proteins / Christian T. Matson, Lester R. Drewes 479

33 Immunohistochemical Detection of Endothelial Proteins / Sukriti Nag 489

Part VI Genetically Altered Mice

34 Cerebral Vascular Function in Genetically Altered Mice / Frank M. Faraci 505

35 Generation of Transgenic Mice by Pronuclear Injection / Annette Damert, Heike Kusserow 513

36 Mouse Genome Modification / Richard Rozmahel 529.

Notes:

Includes bibliographical references and index.

ISBN:

1588290735

OCLC:

51607864