Human embryonic stem cells / edited by Arlene Chiu and Mahendra S. Rao.

Format:

Book

Contributor:

Chiu, Arlene Y.

Rao, Mahendra S.

Language:

English

Subjects (All):

Human embryo.

Embryonic stem cells.

Embryo Research.

Stem Cells.

Medical Subjects:

Embryo Research.

Stem Cells.

Physical Description:

xviii, 461 pages : illustrations ; 23 cm

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

Although there is great interest in the potential for using stem cells as cell replacements and other treatments for diseases that currently have no cure, research on the biology of human embryonic stem cells is still in its infancy. In Human Embryonic Stem Cells, pioneers, leaders, and experts in this emerging field join forces to address all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, these topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. The authors cover the criteria used by investigators in different fields to recognize mature phenotypes of specific tissues. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property. Reviewing the most pressing issues involved in human embryonic stem cell research, Human Embryonic Stem Cells, provides an invaluable sourcebook for researchers seeking a review of their basic biology and an unbiased assessment of their potential for new therapies.

Contents:

Part I Policy

1 Ethical Issues Associated with Pluripotent Stem Cells / Steve Usdin 3

2 A Researcher's Guide to Federally Funded Human Embryonic Stem Cell Research in the United States / Gregory J. Downing 27

3 Intellectual Property of Human Pluripotent Stem Cells / Mark L. Rohrbaugh 39

Part II Types of Pluripotent Cells

4 Embryonal Carcinoma Cells: The Malignant Counterparts of ES and EG Cells / Jonathan S. Draper, Harry Moore, Peter W. Andrews 63

5 Human Pluripotent Stem Cells from Bone Marrow / Felipe Prosper, Catherine M. Verfaillie 89

6 Protocols for the Isolation and Maintenance of Human Embryonic Stem Cells / Melissa K. Carpenter, Chunhui Xu, Christine A. Daigh, Jessica E. Antosiewicz, James A. Thomson 113

7 Subcloning and Alternative Methods for the Derivation and Culture of Human Embryonic Stem Cells / Michal Amit, Hanna Segev, Dorit Manor, Joseph Itskovitz-Eldor 127

Part III Differentiation

8 Differentiation of Neuroepithelia from Human Embryonic Stem Cells / Su-Chun Zhang 145

9 Pancreatic Differentiation of Pluripotent Stem Cells / Nadya Lumelsky 161

10 Human Embryonic Stem Cell-Derived Cardiomyocytes: Derivation and Characterization / Chunhui Xu, Melissa K. Carpenter 181

11 Vascular Lineage Differentiation from Human Embryonic Stem Cells / Sharon Gerecht-Nir, Joseph Itskovitz-Eldor 201

12 Hematopoietic Progenitors Derived from Human Embryonic Stem Cells / Dan S. Kaufman 219

Part IV Therapeutics

13 Human Embryonic vs Adult Stem Cells for Transplantation Therapies / Calvin B. Harley, Mahendra S. Rao 239

14 Genetic Manipulation of Human Embryonic Stem Cells / Micha Drukker, Nissim Benvenisty 265

15 Human Therapeutic Cloning / Jose B. Cibelli 285

16 Therapeutic Uses of Embryonic Stem Cells / Alexander Kamb, Mani Ramaswami, Mahendra S. Rao 297

17 Human Embryonic Stem Cells and the Food and Drug Administration: Assuring the Safety of Novel Cellular Therapies / Donald W. Fink, Jr. 323

18 Studies of a Human Neuron-Like Cell Line in Stroke and Spinal Cord Injury: Preclinical and Clinical Perspectives / Paul J. Reier, John Q. Trojanowski, Virginia M-Y. Lee, Margaret J. Velardo 345

Appendix I Cell Lines and Companies Involved with Human Embryonic Stem Cell Research 389

Appendix III Research Agreements and Material Transfer Agreements Between Investigator and Stem Cell Provider 399

Appendix IV Stem Cell Patents 413.

Notes:

Includes bibliographical references and index.

ISBN:

1588293114

OCLC:

51637856