Recombinant listeria monocytogenes as a live oral delivery vector of SIV GAG protein / Ross Stephen Johnson.

Format:

Book

Manuscript

Microformat

Thesis/Dissertation

Author/Creator:

Johnson, Ross Stephen.

Contributor:

Paterson, Yvonne, 1941- advisor.

University of Pennsylvania.

Language:

English

Subjects (All):

Penn dissertations--Immunology.

Immunology--Penn dissertations.

Allergy and Immunology.

Academic Dissertations as Topic.

Medical Subjects:

Allergy and Immunology.

Academic Dissertations as Topic.

Local Subjects:

Penn dissertations--Immunology.

Immunology--Penn dissertations.

Physical Description:

xvi, 120 pages : illustrations (some color) ; 29 cm

Production:

2003.

Summary:

Listeria monocytogenes is a gram-positive, non-spore-forming bacterium. It has been used as an experimental live vector for the induction of antigen specific immune responses in various experimental models. Most of these studies have only looked at the use of L. monocytogenes given either i.v. or i.p. However, the ability of L. monocytogenes to naturally infect via the oral route suggests that it could be a good mechanism for the oral delivery of passenger antigens. The Human Immunodeficiency Virus, HIV, is the causative agent of AIDS which causes a great loss of human life and health care resources. The non-human primate model of this illness is the Simian Immunodeficiency Virus, which produces an AIDS like disease in certain monkey species. A recombinant strain of Listeria monocytogenes , which expresses and secretes the Simian Immunodeficiency Virus Gag protein (Lm-SIV-Gag) was developed and used as a live oral delivery vector. To test the ability of Lm-SIV-Gag to induce immunity to the SIV Gag antigen, BALB/c mice were immunized orally or parenterally and their cellular and humoral immune responses to SIV Gag were measured. Lm-SIV-Gag induced a measurable SIV Gag specific CTL responses and SIV Gag specific proliferation, but failed to induce a SIV Gag specific humoral response. Spleen cells and Mesenteric Lymph Node cells collected after oral and parenteral inoculation showed SIV p27 Gag-specific IFN-gamma responses after restimulation with SIV p27 Gag, which could be attributed to CD4+ T cells. These studies show that Lm-SIV-Gag induces predominately a Th1 type response and cell mediated immunity whether delivered parenterally or orally.

Notes:

Adviser: Yvonne Paterson.

Thesis (Ph.D. in Immunology) -- University of Pennsylvania, 2003.

Includes bibliographical references.

Local Notes:

University Microfilms order no.: 3095894.

OCLC:

244973012