Molecular biology in cellular pathology / edited by John Crocker, Paul G. Murray.

Format:

Book

Contributor:

Crocker, J.

Murray, Paul (Paul G.)

Language:

English

Subjects (All):

Pathology, Molecular.

Pathology, Cellular.

Genetic Techniques.

Cell Physiological Phenomena.

Cells--pathology.

Medical Subjects:

Genetic Techniques.

Cell Physiological Phenomena.

Cells--pathology.

Physical Description:

xx, 380 pages, 8 unnumbered pages of plates : illustrations (some color) ; 25 cm

Edition:

Second edition.

Place of Publication:

Chichester, England ; Hoboken, NJ : John Wiley & Sons, [2003]

Summary:

Molecular biology has had a major impact on the practice of pathology in recent years and this continues with the new approaches being developed in genomics and proteomics. Knowledge of the molecular organization of the cell has led to the development of powerful new techniques which bring greater accuracy and objectivity to the diagnosis, prognosis and management of many diseases, and to the study of pathological states. This book is a completely revised and updated version of the popular Molecular Biology in Histopathology, with added material covering the latest methodologies and now featuring colour plates. It describes the molecular techniques available and provides an overview of how they can be applied. It is aimed at histopathologists in training, clinicians and biologists studying for post-graduate qualifications and will also be of interest to biochemists, physiologists, pharmacologists and biotechnologists, in academia, clinical medicine and industry.

Contents:

1 Blotting Techniques: Methodology and Applications / Fiona Watson, C. Simon Herrington 1

1.2 Blotting techniques 1

2 In-situ Hybridisation in Histopathology / Gerald Niedobitek, Hermann Herbst 19

2.2 Experimental conditions 20

2.3 Probes and labels 23

2.4 Controls and pitfalls 27

2.5 Double-labelling 29

2.6 Increasing the sensitivity of ISH 31

2.7 What we do in our laboratories 33

2.8 Applications of ISH: examples 35

2.9 Perspective 39

3 DNA Flow Cytometry / M.G. Ormerod 49

3.2 Definitions and terms 49

3.3 Dye used for DNA analysis 50

3.4 Sample preparation for DNA analysis 52

3.5 Analysis of the DNA histogram 53

3.6 Quality control 53

3.7 Computer analysis of the DNA histogram 55

3.8 Multiparametric measurement 57

4 Interphase Cytogenetics / Sara A. Dyer, Jonathan J. Waters 61

4.2 Interphase cytogenetics 62

4.3 Applications 67

5 Oncogenes / Fiona Macdonald 79

5.2 Identification of the oncogenes 79

5.3 Functions of the proto-oncogenes 80

5.4 Mechanism of oncogene activation 89

5.5 Oncogenes in colorectal cancer 91

5.6 Oncogenes in breast cancer 94

5.7 Oncogenes in lung cancer 95

5.8 Oncogenes in haematological malignancies 96

5.9 Other cancers 99

6 Molecular and Immunological Aspects of Cell Proliferation / Karl Baumforth, John Crocker 105

6.1 The cell cycle and its importance in clinical pathology 105

6.2 Molecular control of the cell cycle 108

6.3 Cell cycle control 111

6.4 The cell cycle and cancer 112

6.5 Immunocytochemical markers of proliferating cells 115

7 Interphase Nucleolar Organiser Regions in Tumour Pathology / Massimo Derenzini, Davide Trere, Marie-Francoise O'Donohue, Dominique Ploton 137

7.2 The AgNORs 138

7.3 NOR silver-staining 142

7.4 Quantitative AgNOR analysis 145

7.5 AgNORs as a parameter of the level of cell proliferation 146

7.6 Application of the AgNOR technique to tumour pathology 147

7.7 What future for AgNORs in tumour pathology? 151

8 Apoptosis and Cell Senescence / Lee B. Jordan, David J. Harrison 153

8.2 Apoptosis 153

8.3 Cell senescence 174

9 The Polymerase Chain Reaction / Timothy Diss 193

9.3 Analysis of products 197

9.4 RT-PCR 199

9.5 Quantitative PCR 200

9.6 DNA and RNA extraction 200

9.7 Correlation of the PCR with morphology 201

9.8 Problems 202

9.9 Applications 202

9.10 Diagnostic applications 203

9.11 Infectious diseases 209

9.12 Identity 209

9.13 The future 210

9.15 Online information 212

10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell / Amanda Dutton, Victor Lopes, Paul G. Murray 213

10.3 Technical considerations 216

10.4 Advantages and disadvantages of LCM 217

10.5 Applications of LCM 222

10.6 Future perspectives 229

11 The In-situ Polymerase Chain Reaction / John J. O'Leary, Cara Martin, Orla Sheils 233

11.3 In-cell PCR technologies 235

11.4 In-cell amplification of DNA 238

11.5 Detection of amplicons 242

11.6 Reaction, tissue and detection controls for use with in-cell DNA PCR assays 243

11.7 In-cell RNA amplification 244

11.8 Problems encountered with in-cell PCR amplification 246

11.9 Amplicon diffusion and back diffusion 247

11.10 Future work with in-cell PCR-based assays 247

12 TaqMan Technology and Real-Time Polymerase Chain Reaction / John J. O'Leary, Orla Sheils, Cara Martin, Aoife Crowley 251

12.2 Probe technologies 252

12.3 TaqMan probe and chemistry (first generation) 254

12.4 Second generation TaqMan probes 256

12.5 Hybridisation 258

12.6 TaqMan PCR conditions 259

12.7 Standards for quantitative PCR 260

12.8 Interpretation of results 261

12.9 End-point detection 262

12.10 Real-time detection 263

12.11 Relative quantitation 263

12.12 Reference genes 264

12.13 Specific TaqMan PCR applications 265

13 Gene Expression Analysis Using Microarrays / Sophie E. Wildsmith, Fiona J. Spence 269

13.2 Microarray experiments 269

13.3 Data analysis 273

13.4 Recent examples of microarray applications 284

14 Comparative Genomic Hybridisation in Pathology / Marjan M. Weiss, Mario A.J.A. Hermsen, Antoine Snijders, Horst Buerger, Werner Boecker, Ernst J. Kuipers, Paul J. van Diest, Gerrit A. Meijer 287

14.2 Technique 289

14.3 Data analysis 292

14.4 Applications 293

14.5 Clinical applications 299

14.6 Screening for chromosomal abnormalities in fetal and neonatal genomes 299

14.7 Future perspectives 300

15 DNA Sequencing and the Human Genome Project / Philip Bennett 307

15.2 DNA sequencing: the basics 308

15.3 Applications of DNA sequencing 318

15.4 The Human Genome Project 320

16 Monoclonal Antibodies: The Generation and Application of 'Tools of the Trade' Within Biomedical Science / Paul N. Nelson, S. Jane Astley, Philip Warren 329

16.2 Antibodies and antigens 331

16.3 Polyclonal antibodies 332

16.4 Monoclonal antibody development 333

16.5 Monoclonal antibody variants 338

16.6 Monoclonal antibody applications 341

16.7 Therapy 345

16.8 Specific applications 346

17 Proteomics / Kathryn Lilley, Azam Razzaq, Michael J. Deery 351

17.2 Definitions and applications 352

17.3 Stages in proteome analysis 352

17.4 Future directions 368.

Notes:

Rev. ed. of: Molecular biology in histopathology, c1994.

Includes bibliographical references and index.

ISBN:

0470844752

OCLC:

51438100