Inflammation protocols / edited by Paul G. Winyard and Derek A. Willoughby.

Format:

Book

Contributor:

Winyard, Paul G., 1959-

Willoughby, D. A.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 225.

Methods in molecular biology ; v. 225

Language:

English

Subjects (All):

Inflammation--Laboratory manuals.

Inflammation.

Inflammation--Mediators--Laboratory manuals.

Anti-inflammatory agents--Laboratory manuals.

Anti-inflammatory agents.

Inflammation--Mediators.

Anti-Inflammatory Agents.

Medical Subjects:

Inflammation.

Anti-Inflammatory Agents.

Genre:

Laboratory manuals.

Physical Description:

xii, 378 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

Because inflammation is involved in many chronic human diseases, its proper understanding may provide important clues to new therapeutic targets. In Inflammation Protocols, highly skilled experimenters present key techniques for the multidisciplinary study of inflammation in such conditions as inflammatory bowel disease, rheumatoid arthritis, renal disease, and cardiovascular disease. Described in step-by-step detail to ensure experimental success, the techniques are focused on the research and development of potential new antiinflammatory drugs in active target areas. The protocols are suitable for many current areas of drug discovery research, including transcription factors, cytokines, adhesion molecules, cyclooxygenase-2 (COX-2) inhibitors, free radicals, nitric oxide synthases, complement activation, angiogenesis, wound healing, immune rejection, and metalloproteinases. The methods provide in vitro systems for studying inflammation, in vivo models, and relevant pharmacodynamic measurements for the assessment of antiinflammatory compounds. Each protocol contains notes about potential pitfalls and tips on how to avoid failure. Overview chapters at the start of each section review the significance and potential limitations of the protocols presented. Cutting-edge and highly practical, Inflammation Protocols offers experimental and clinical researchers a premier collection of readily reproducible techniques not only for evaluating inflammatory disease processes, but also for the discovery of new drugs for treating chronic human inflammatory diseases today.

Contents:

Part I. In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs

1. Key Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets: Introduction to Part 1 / Paul G. Winyard 3

2. I[kappa]B Kinase and NF-[kappa]B Signaling in Response to Pro-Inflammatory Cytokines / Mireille Delhase 7

3. Screening for Inhibitors of Transcription Factors Using Luciferase Reporter Gene Expression in Transfected Cells / Deborah Phippard, Anthony M. Manning 19

4. Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells / Susan L. Cuvelier, Kamala D. Patel 25

5. Phagocytosis by Inflammatory Phagocytes: Experimental Strategies for Stimulation and Quantification / M. Rachel Morris, Sharon Dewitt, Iraj Laffafian, Maurice B. Hallett 35

6. Cytosolic Ca[superscript 2+] Measurement and Imaging in Inflammatory Cells / Sharon Dewitt, Iraj Laffafian, M. Rachel Morris, Maurice B. Hallett 47

7. Detection and Visualization of Oxidase Activity in Phagocytes / Maurice B. Hallett, Caroline Cole, Sharon Dewitt 61

8. Measurement of Complement Activation / Tom Eirik Mollnes 69

9. Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography / Linda Troeberg, Hideaki Nagase 77

10. Measurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot Analysis / Clare E. Hughes, Christopher B. Little, Bruce Caterson 89

11. In Vitro Model of Human Articular Cartilage Degradation / William D. Shingleton 99

Part II. In Vivo Models of Inflammation

12. In Vivo Models of Inflammation: Introduction to Part 2 / Derek A. Willoughby 109

13. Carrageenan-Induced Paw Edema in the Rat and Mouse / Christopher J. Morris 115

14. Pleural Models of Inflammation: Immune and Nonimmune / Adrian R. Moore 123

15. Models of Acute Inflammation in the Ear / Miklos Gabor 129

16. Migration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In Vivo / Mauro Perretti, Stephen J. Getting 139

17. Inflammatory Joint Disease: Clinical, Histological, and Molecular Parameters of Acute and Chronic Inflammation and Tissue Destruction / Nancy L. McCartney-Francis, James Chan, Sharon M. Wahl 147

18. The Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the Rat / Michael P. Seed 161

19. Collagen-Induced Arthritis / Adrian R. Moore 175

20. Air-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage Degradation / Paul Colville-Nash, Toby Lawrence 181

21. Quantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air Pouch / Chandan A. S. Alam 191

22. Models of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the Heart / Nicole S. Wayman, Michelle C. McDonald, Prabal K. Chatterjee, Christoph Thiemermann 199

23. Assessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel Disease / Brendan J. R. Whittle, Maryan Cavicchi, Dominique Lamarque 209

24. An In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the Rat / Prabal K. Chatterjee, Christoph Thiemermann 223

25. In Vivo Models of Inflammation: Immune Rejection and Skin Transplantation In Vivo / Isabelle Binet, Kathryn J. Wood 239

26. Wound Healing: A Model of Dermal Wound Repair / Annette Tomlinson, Mark W. J. Ferguson 249

Part III. Pharmacodynamic Endpoints in Experimental Models and in Clinical Studies in Humans

27. An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans: Introduction to Part 3 / David R. Blake, Gordon J. Taylor 263

28. A Reply to "An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans" / Derek A. Willoughby 269

29. Quantifying Inflammation In Vivo Using Radiolabeled Antibodies and Leukocytes / Diane Marshall, Dorian O. Haskard 273

30. Immunoperoxidase Histochemistry for the Detection of Cellular Adhesion Molecule, Cytokine, and Chemokine Expression in the Arthritic Synovium / Zoltan Szekanecz, Alisa E. Koch 283

31. Roles of Nitric Oxide and Superoxide in Inflammation / Daniela Salvemini, Harry Ischiropoulos, Salvatore Cuzzocrea 291

32. Analysis of Nitrite and Nitrate in the Study of Inflammation / Claire A. Davies, Sophie A. Rocks, Meg C. O'Shaughnessy, David Perrett, Paul G. Winyard 305

33. In Vivo Assays for COX-2 / Chi-Chung Chan 321

34. Measurement of 8-epi-PGF[subscript 2[alpha] as a Marker of Lipid Peroxidation In Vivo by Immunoaffinity Extraction and Gas Chromatography-Mass Spectrometry / Nitin K. Gopaul, Erik E. Anggard 329

35. Laboratory Assessment of the Acute Phase Response: Using CRP as a Model / Robert F. Ritchie, Thomas B. Ledue 343

36. Assays of Matrix Metalloproteinases (MMPs) and MMP Inhibitors: Bioassays and Immunoassays Applicable to Cell Culture Medium, Serum, and Synovial Fluid / Jon B. Catterall, Tim E. Cawston 353.

Notes:

Includes bibliographical references and index.

ISBN:

0896039706

OCLC:

50684651