Prokaryotic genomics / edited by Michel Blot.

Format:

Book

Contributor:

Blot, Michel.

Series:

Methods and tools in biosciences and medicine

Language:

English

Subjects (All):

Bacterial genetics--Laboratory manuals.

Bacterial genetics.

Genomics--Laboratory manuals.

Genomics.

Proteomics--Laboratory manuals.

Proteomics.

Genetics, Microbial.

Medical Subjects:

Genetics, Microbial.

Genomics.

Proteomics.

Genre:

Laboratory manuals.

Physical Description:

xii, 208 pages : illustrations (some color), portrait ; 26 cm.

Place of Publication:

Basel ; Boston : Birkhäuser Verlag, [2003]

Summary:

This book is part of the Methods and Tools in Biosciences and Medicine series, representing a new standard of comprehensive laboratory manuals for researchers and workers at all levels and in various fields of biology and medicine. This book includes detailed information from basic theory to sources of equipment and reagents. It covers background information, detailed step-by-step protocols, typical data, hints and tips for success, troubleshooting guides and key literature references.

Contents:

1 Physical Analysis of Chromosome Size Variation / Colin Dale, Wendy Smith, Howard Ochman 1

2 Genetic Mapping in Salmonella enterica / Josep Casadesus, Eva M. Camacho 10

3 Insertion Sequences as Genomic Markers / Dominique Schneider, Michel Blot 22

4 The Use of Noncoding Microsatellite Length Analysis for Bacterial Strain Typing / David Metzgar 34

5 How to Amplify Easily, on the Bacterial Chromosome, a Desired DNA Sequence / Richard D'Ari, Daniel Vinella 41

6 Generalized Transduction / Anne Thierauf, Stanley Maloy 50

7 Use of Conditional-replication, Integration, and Modular CRIM Plasmids to Make Single-copy lacZ Fusions / Lu Zhou, Soo-Ki Kim, Larisa Avramova, Kirill A. Datsenko, Barry L. Wanner 65

8 Genetic Footprinting for Bacterial Functional Genomics / Scott S. Walker, Chad Houseweart, Teresa J. Kenney 90

9 Gene Transfer to Plants through Bacterial Vectors / Bruno Tinland 102

10 Quorum Sensing: Approaches to Identify Signals and Signalling Genes in Gram-negative Bacteria / Simon Swift 110

11 Transcriptional Profiling in Bacteria Using Microarrays / Michael T. Laub, R. Frank Rosenzweig 131

12 Transcriptome Analysis by Macroarrays / Cecile Jourlin-Castelli, Francois Denizot, Philippe Bouloc 145

13 Prokaryotic Proteomics / Cecile Lelong, Thierry Rabilloud 157

14 Intein-mediated Protein Purification / Shaorong Chong, Francine B. Perler 172

15 Two-hybrid Assay in Escherichia coli K12 / Gustavo Di Lallo, Patrizia Ghelardini, Luciano Paolozzi 194.

Notes:

Includes bibliographical references and indexes.

ISBN:

3764365978

376436596X

OCLC:

50253162