Pocket guide to biotechnology and genetic engineering / Rolf D. Schmid.

Format:

Book

Author/Creator:

Schmid, Rolf D., 1942-

Language:

English

Subjects (All):

Biotechnology.

Genetic engineering.

Physical Description:

x, 350 pages : color illustrations, portrait ; 19 cm

Place of Publication:

Weinheim [Germany] : Wiley-VCH, [2003]

Summary:

Biotechnology and genetic engineering are the key technologies of the 21st century. They allow the findings in cell biology and genetics, biochemistry and microbiology, biochemical engineering and bioinformatics to be applied to health care, agriculture, food production, environmental protection and alternative production methods for chemicals. This pocket guide provides a broad overview of the relevant facts on products, methods and applications, while discussing the opportunities and risks of these new technologies as well as ethical, economic and safety considerations. The whole is backed by instructive yet attractive color illustrations and an excellent didactic approach throughout. A perfect introduction to the field - for professionals and students alike.

Contents:

Historical survey

Early developments 2

Biotechnology today 4

Food biotechnology

Alcoholic beverages 6

Beer 8

Fermented food 10

Food and lactic acid fermentation 12

Alcohols, acids, and amino acids Ethanol 14

1-Butanol, acetone 16

Acetic acid/vinegar 18

Citric acid 20

Lactic acid, gluconic acid 22

Amino acids 24

L-Glutamic acid 26

D,L-Methionine, L-lysine, and L-threonine 28

Aspartame, L-phenylalanine, and L-aspartic acid 30

Amino acids via enzymatic transformation 32

Antibiotics

Antibiotics: occurrence, applications, mechanism of action 34

Antibiotics: industrial production, resistance 36

[beta]-Lactam antibiotics: structure, biosynthesis and mechanism of action 38

[beta]-Lactam antibiotics: manufacture 40

Amino acid and peptide antibiotics 42

Glycopeptide, polyether, and nucleoside antibiotics 44

Aminoglycoside antibiotics 46

Tetracyclines, chinones, chinolones, and other aromatic antibiotics 48

Macrolide antibiotics 50

New pathways to antibiotics 52

Specialties

Vitamins 54

Nucleosides and nucleotides 56

Biosurfactants and biocosmetics 58

Microbial polysaccharides 60

Biomaterials 62

Biotransformation 64

Steroid biotransformations 66

Enzymes

Enzyme catalysis 70

Analytical enzymes 72

Enzyme tests 74

Enzymes as additives 76

Detergent enzymes 78

Enzymes for starch hydrolysis 80

Enzymatic starch hydrolysis 82

Enzymes and sweeteners 84

Enzymes for the hydrolysis of cellulose and polyoses 86

Enzymes in pulp and paper processing 88

Pectinases 90

Enzymes and milk products 92

Enzymes in baking and meat processing 94

Enzymes in leather and textile treatment 96

Procedures for obtaining novel technical enzymes 98

Bakers' yeast and single cell technologies

Bakers' yeast and fodder yeasts 100

Single cell protein, single cell oil 102

Biotechnology and environmental processes

Aerobic wastewater treatment 104

Anaerobic wastewater and sludge treatment 106

Biological treatment of exhaust air 108

Biological soil treatment 110

Microbial leaching, biofilms, and biocorrosion 112

Medical biotechnology

Insulin 114

Growth hormone and other hormones 116

Hemoglobin, serum albumen, and lactoferrin 118

Blood clotting agents 120

Anticoagulants and thrombolytic agents 122

Enzyme inhibitors 124

The immune system 126

Stem cells 128

Tissue Engineering 130

Interferons 132

Interleukins 134

Erythropoietin and other growth factors 136

Other therapeutic proteins 138

Vaccines 140

Recombinant vaccines 142

Antibodies 144

Monoclonal antibodies 146

Recombinant and catalytic antibodies 148

Immunoanalysis 150

Biosensors 152

Agricultural biotechnology

Animal breeding 154

Embryo transfer, cloned animals 156

Gene maps 158

Transgenic animals 160

Gene farming and xenotransplantation 162

Plant breeding 164

Plant tissue surface culture 166

Plant cell suspension culture 168

Transgenic plants: methods 170

Transgenic plants: resistance 172

Transgenic plants: products 174

Microbiological fundamentals

Viruses 176

Bacteriophages 178

Microorganisms 180

Bacteria 182

Some bacteria of importance for biotechnology 184

Fungi 186

Yeasts 188

Microorganisms: isolation, preservation, safety 190

Microorganisms: strain improvement 192

Bioengineering fundamentals

Growing microorganisms 194

Growth kinetics and product formation 196

Fed-batch and continuous fermentation 198

Fermentation technology 200

Fermentation technology: scale-up 202

Cultivation of mammalian cells 204

Mammalian cell bioreactors 206

Enzyme and cell reactors 208

Recovery of bioproducts 210

Recovery of proteins: chromatography 212

Economic aspects of industrial processes 214

Fundamentals of molecular genetics

DNA: structure 216

DNA: function 218

Genetic engineering: general steps 220

Preparation of DNA 222

Other useful enzymes for DNA manipulation 224

PCR: general method 226

PCR: laboratory methods 228

DNA: synthesis and size determination 230

DNA sequencing 232

Transfer of foreign DNA in living cells (transformation) 234

Gene cloning and identification 236

Gene expression 238

Gene silencing 240

RNA 242

Gene libraries and gene mapping 244

Genetic maps of prokaryotes 246

Genetic maps of eukaryotes 248

The human genome 250

Functional analysis of the human genome 252

Recent trends

DNA assays 254

DNA and protein arrays 256

Reporter groups 258

Protein design 260

Gene therapy 262

Proteomics 264

Drug screening 266

Bioinformatics 268

Metabolism 270

Metabolic engineering 272

Systems biology 274

Safety, ethics, economic issues

Safety in genetic engineering 276

Regulation of products derived from biotechnology 278

Ethical considerations and acceptance 280

Patents in biotechnology 282

International aspects of biotechnology 284

Literature 319

Origin of materials 345.

Notes:

Includes bibliographical references (pages 319-343) and index.

ISBN:

3527308954

OCLC:

50645336