Cancer cell signaling : methods and protocols / edited by David M. Terrian.

Format:

Book

Contributor:

Terrian, David M.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 218.

Methods in molecular biology ; 218

Language:

English

Subjects (All):

Cancer cells--Regulation--Laboratory manuals.

Cancer cells.

Cellular signal transduction--Laboratory manuals.

Cellular signal transduction.

Cancer--Genetic aspects--Laboratory manuals.

Cancer.

Neoplasms--physiopathology.

Cancer--Genetic aspects.

Neoplasms--genetics.

Signal Transduction--physiology.

Medical Subjects:

Neoplasms--physiopathology.

Neoplasms--genetics.

Signal Transduction--physiology.

Genre:

Laboratory manuals.

Physical Description:

xiii, 333 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2003.

Summary:

The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid.

Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.

Contents:

Part I. Manipulation and Detection of Survival Signals

1 Functional Analysis of the Antimitogenic Activity of Tumor Suppressors / Erik S. Knudsen, Steven P. Angus 3

2 Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination / Simon W. Hayward, Yuzhuo Wang, Mark L. Day 17

3 Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells / Hideki Kawasome, Takashi Hamazaki, Tetsuo Minamino, Naohiro Terada 35

4 The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress / Richard A. Franklin 47

5 Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression / Christopher W. Gregory 59

6 Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells / Raymond R. Mattingly 71

7 TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s) / Stephanie M. Oberhaus 85

Part II. Manipulation and Detection of Oncogenic Signals

8 Kinetworks Protein Kinase Multiblot Analysis / Steven Pelech, Catherine Sutter, Hong Zhang 99

9 Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers / Wen-Chang Lin 113

10 Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay / Yan-Hua Chen, Qun Lu 127

11 Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay / Irwin H. Gelman 133

12 Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells / Daqing Wu, Ginger G. Wescott, David M. Terrian 143

13 Measurements of Phospholipases A[subscript 2], C, and D (PLA[subscript 2], PLC, and PLD): In Vitro Microassays, Analysis of Enzyme Isoforms, and Intact-Cell Assays / Julian Gomez-Cambronero, Joel Horwitz, Ramadan I. Sha'afi 155

14 Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model / Yunfeng Zhao, Daret K. St. Clair 177

15 Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer / Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton, Patrick M. Navolanic, Julianne M. Davis, Steven L. Knapp, Richard A. Franklin, Martyn K. White, James A. McCubrey 185

16 Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes / Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton, Patrick M. Navolanic, Julianne M. Davis, Steven L. Knapp, Richard A. Franklin, Martyn K. White, James A. McCubrey 203

17 Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes / Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton, Patrick M. Navolanic, Julianne M. Davis, Steven L. Knapp, Richard A. Franklin, Martyn K. White, James A. McCubrey 221

Part III. Protein Interactions

18 Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology / Daniel Price, Iha Park, Hava Avraham 255

19 Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay / Andre Nantel 269

20 Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions / Lucio Comai 277

Part IV. Genomic Rearrangements

21 Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing / Sheila Greene, Patricia Stockton, Olga G. Kozyreva, Kris E. Gaston, Gordon P. Flake, James L. Mohler 287

22 Clonality Analysis by T-Cell Receptor [gamma] PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL) / Ansgar Lukowsky 303.

Notes:

Includes bibliographical references and index.

ISBN:

1588290751

OCLC:

50124823