Superantigen protocols / edited by Teresa Krakauer.

Format:

Book

Contributor:

Krakauer, Teresa.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 214.

Methods in molecular biology ; 214

Language:

English

Subjects (All):

Superantigens--Laboratory manuals.

Superantigens.

Clinical Laboratory Techniques.

Medical Subjects:

Superantigens.

Clinical Laboratory Techniques.

Genre:

Laboratory manuals.

Physical Description:

xii, 259 pages ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2003.

Summary:

Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level.

Contents:

1 Superantigens: Structure, Function, and Diversity / Matthew D. Baker, K. Ravi Acharya 1

2 Expression, Purification, and Detection of Novel Streptococcal Superantigens / John K. McCormick, Patrick M. Schlievert 33

3 Flow Cytometric Detection of MMTV Superantigens / Gary Winslow 45

4 Spectrophotometric Methods for the Determination of Superantigen Structure and Stability / Anders Cavallin, Karin Petersson, Goran Forsberg 55

5 Binding Kinetics of Superantigen with TCR and MHC Class II / S. Munir Alam, Nicholas R. J. Gascoigne 65

6 Directed Evolution of T-Cell Receptors for Binding Superantigens / Hywyn R. O. Churchill, David M. Kranz 87

7 Analysis of Superantigen Binding to Soluble T-Cell Receptors / Hywyn R. O. Churchill, David M. Kranz 101

8 Role of Accessory Molecules in the Superantigen-Induced Activation of Peripheral Blood T Cells / Catherine Gelin, Marie-Therese Zilber, Dominique Charron 113

9 T-Lymphocyte Activation Induced by Staphylococcal Enterotoxin Superantigens: Analysis of Protein Tyrosine Phosphorylation / Anne Roumier, Florence Niedergang, Andres Alcover 127

10 Measurement of Proinflammatory Cytokines and T-Cell Proliferative Response in Superantigen-Activated Human Peripheral Blood Mononuclear Cells / Teresa Krakauer 137

11 RNase Protection Assay for the Study of the Differential Effects of Therapeutic Agents in Suppressing Staphylococcal Enterotoxin B-Induced Cytokines in Human Peripheral Blood Mononuclear Cells / Teresa Krakauer, Xin Chen, O. M. Zack Howard, Howard A. Young 151

12 Staining Protocol for Superantigen-Induced Cytokine Production Studied at the Single-Cell Level / Lars Bjork 165

13 Assessment of Specific T-Cell Activation by Superantigens / Natalie Sutkowski, Brigitte T. Huber 185

14 Superantigen-Induced Changes in Epithelial Ion Transport and Barrier Function: Use of an In Vitro Model / James L. Watson, Derek M. McKay 219

15 Pyrogenic, Lethal, and Emetic Properties of Superantigens in Rabbits and Primates / John K. McCormick, Gregory A. Bohach, Patrick M. Schlievert 245.

Notes:

Includes bibliographical references and index.

ISBN:

0896039846

OCLC:

49322437