Cytokines and colony stimulating factors : methods and protocols / edited by Dieter Körholz and Wieland Kiess.

Format:

Book

Contributor:

Körholz, Dieter.

Kiess, W. (Wieland)

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 215.

Methods in molecular biology ; v. 215

Language:

English

Subjects (All):

Cytokines--Laboratory manuals.

Cytokines.

Colony-stimulating factors (Physiology)--Laboratory manuals.

Colony-stimulating factors (Physiology).

Colony-Stimulating Factors--physiology.

Medical Subjects:

Cytokines.

Colony-Stimulating Factors--physiology.

Genre:

Laboratory manuals.

Physical Description:

xv, 478 pages : illustrations ; 23 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

Hands-on laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Although the protocols require little previous experience, even highly skilled researchers will augment their skills with many novel timesaving techniques.

Contents:

1 Primary Immunodeficiencies Caused by Defects of Cytokines and Cytokine Receptors / Volker Wahn 3

Part II. Detection Assays for Cytokines and Growth Factors

2 Intracellular Detection of T-Cell Cytokines: Differentiation of TH1 and TH2 Cells / Ursula Banning, Dieter Korholz 15

3 Analysis of Cytokine Profiles in Human Skin / Jean Krutmann, Renz Mang, Susanne Grether-Beck 23

4 Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry / Christian Schultz 29

5 Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells / Kerstin Willmann 41

6 Evaluation of the Frequency of Virus-Specific CD8+ T Cells by Cytokine Flow Cytometry / Bodo Hoffmeister, Felix Kiecker, Ingolf Surel, Elham Khatamzas, Volker Schuster, Hans-Dieter Volk, Florian Kern 59

7 Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms / Thomas Lehrnbecher, Stephen J. Chanock 71

8 Apoptosis Induction by TRAIL / Angelika Eggert, Hauke Sieverts, Henning Walczak 95

9 Angiogenic Cytokines: Quantitative and Functional Analysis / Angelika Eggert, John H. Maris 117

Part III. Protocols on Gene Therapy

10 Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy: An Overview / Christof M. Kramm, Tim Niehues, Nikolai G. Rainov 137

11 Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins / Ciriaco A. Piccirillo, Gerald J. Prud'homme 153

12 Interferon-beta Gene Therapy for the Treatment of Arthritis / Yuti Chernajovsky, Hanna Dreja, Kostas Triantaphyllopoulos, David Gould 171

13 Gene Gun-Based In Vivo Gene Transfer: Application to DNA Vaccination / Tohru Sakai, Kunisuke Himeno 181

14 Purification of the Eukaryotic Heat-Shock Proteins Hsp70 and gp96 / Arne von Bonin, Solveig H. More, Minka Breloer 193

15 Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-alpha Moiety / Jim Xiang, John R. Gordon 201

16 Genetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-alpha Gene Transfer / Jim Xiang, Josh Wu 213

17 Retroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood Lymphocytes / Timothy M. Clay, Michael I. Nishimura 227

18 Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus / Glyn D. Palmer, Elvire Gouze, Jean-Noel Gouze, Oliver B. Betz, Christopher H. Evans, Steven C. Ghivizzani 235

19 Gene Therapy of X-Linked Severe Combined Immunodeficiency / Salima Hacein-Bey-Abina, Genevieve de Saint Basile, Marina Cavazzana-Calvo 247

20 Gene Transfer for Generation of Tumor and Leukemia Vaccines / Dagmar Dilloo, Andree Zibert 261

21 Cytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral Vectors / Roberto Furlan, Stefano Pluchino, Peggy C. Marconi, Gianvito Martino 279

Part IV. Ex Vivo Cell Expansion

22 In Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative Disease / Thomas Vraetz, Peter D. Emanuel, Charlotte M. Niemeyer 293

23 In Vitro Generation of Dendritic Cells from Cord Blood CD34+ Hematopoietic Progenitors Cells / Bruno Canque, Michele Rosenzwajg, Jean Claude Gluckman 311

24 Generation of Transgenic T Cells from Human CD34+ Cord Blood Cells / Bruno Verhasselt, Evelien Naessens, Veronique Stove 327

25 Ex Vivo Expansion of Umbilical Cord Blood Cells on Feeder Layers / Martin Bornhauser 341

26 Ex Vivo Production of Cord Blood CD34+ Derived Myeloid Precursors After Serum-Free Static Culture / Sergio Querol 351

27 Ex Vivo Expansion of Hematopoietic Stem Cells / Beatriz Albella, Jose Carlos Segovia, Guillermo Guenechea, Juan Antonio Bueren 363

28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use / Phil Lefebvre, Isaac Cohen 375

29 Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells / Kohichiro Tsuji, Takahiro Ueda, Yasuhiro Ebihara 387

Part V. Maturation and Differentiation of Dendritic Cells

30 Differentiation of Human Antigen-Presenting Dendritic Cells from CD34+ Hematopoietic Stem Cells In Vitro / Xin-Sheng Ju, Martin Zenke 399

31 Dendritic Cell Development from Mobilized Peripheral Blood CD34+ Cells / Deanne M. R. Lathers, Nicholas Achille, M. Rita I. Young 409

32 In Vitro Maturation of Dendritic Cells from Blood Progenitors / Lina Matera, Alessandra Galetto 417

33 Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood / Pawel Kalinski, Pedro Vieira, Joost H. N. Schuitemaker, Quan Cai, Martien Kapsenberg 427

34 Preparation of Human Dendritic Cells for Tumor Vaccination / Michael R. Shurin 437

35 Generation of Leukemic Dendritic Cells from Patients with Acute Myeloid Leukemia / Mohamad Mohty, Beatrice Gaugler, Daniel Olive 463.

Notes:

Includes bibliographical references and index.

ISBN:

1588290352

OCLC:

50198799