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Protocols for neural cell culture / edited by Sergey Fedoroff and Arleen Richardson.
Holman Biotech Commons QP356.25 .P7651 2001
Available
- Format:
- Book
- Language:
- English
- Subjects (All):
- Nerve tissue--Cultures and culture media--Laboratory manuals.
- Nerve tissue.
- Neurons--Growth--Laboratory manuals.
- Neurons.
- Nerve Tissue--cytology.
- Neurons--Growth.
- Nerve tissue--Cultures and culture media.
- Neurons--cytology.
- Tissue Culture--methods.
- Medical Subjects:
- Nerve Tissue--cytology.
- Neurons--cytology.
- Tissue Culture--methods.
- Genre:
- Laboratory manuals.
- Physical Description:
- xxii, 362 pages : illustrations ; 28 cm
- Edition:
- Third edition.
- Place of Publication:
- Totowa, N.J. : Humana Press, 2001.
- Summary:
- Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 new chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as are essential procedures for hippocampal and microglial slice cultures. Protocols for Neural Cell Culture: Third Edition is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture and its disorders.
- Contents:
- 1 In Vitro Assays for Axonal Growth and Targeting / Valerie Castellani, Jurgen Bolz 1
- 2 Interface Organotypic Hippocampal Slice Cultures / Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, Luc Stoppini 13
- 3 Slice Cultures for Study of Microglia / Carol A. Colton, Meggan Czapiga, Toby N. Behar 29
- 4 Microexplant Cultures of the Cerebellum / Bernard Rogister, Gustave Moonen 39
- 5 Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons / Robert B. Campenot, Grace Martin 49
- 6 Chick Spinal Somatic Motoneurons in Culture / Bernhard H. J. Juurlink 59
- 7 Primary Cultures of Sympathetic Ganglia / Mary I. Johnson 71
- 8 Primary Cell Cultures for the Study of Myelination / Mary I. Johnson, Richard P. Bunge, Patrick M. Wood 95
- 9 Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures / Ruth Cole, Jean de Vellis 117
- 10 Culture of Glial Cells from Human Brain Biopsies / V. Wee Yong, Jack P. Antel 129
- 11 Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium / Sergey Fedoroff, Arleen Richardson 139
- 12 Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium / Bernhard H. J. Juurlink, Shawn K. Thorburne, Richard M. Devon 149
- 13 Olfactory Ensheathing Cell Cultures / Ronald Doucette 161
- 14 Cultures of Stem Cells of the Central Nervous System / Angela Gritti, Rossella Galli, Angelo Luigi Vescovi 173
- 15 Aggregating Neural Cell Cultures / Paul Honegger, Florianne Monnet-Tschudi 199
- 16 Neural Cell Lines / Kaoru Murayama, Natalia N. Singh, Angela Helmrich, David W. Barnes 219
- 17 Virus Vectors for Gene Therapy of the Nervous System / Sonia N. Yeung, Frank Tufaro 229
- 18 Preparation of Substrata for In Vitro Culture of Neurons / Paul C. Letourneau 245
- 19 Serum-Fee Media for Neural Cell Cultures: Adult and Embryonic / Paul J. Price, Gregory J. Brewer 255
- 20 Development of Multiple-Well Plate Biological Assays / John W. Harbell 265
- 21 Neural Cell Counting: Cell Number Determination in Microplate Cultures / Marston Manthorpe 277
- 22 Assays for Neuronal Cell Migration / Toby N. Behar 291
- 23 Colony Culture: Plating Efficiency Assay and Cloning / Sergey Fedoroff, Arleen Richardson 307
- 24 Hybridoma Cultures for Production of Antibodies: Purification and Specificity of Antibodies / J. Denry Sato 317
- 25 Elimination of Cell Types from Mixed Neural Cell Cultures / Richard M. Devon 325
- 26 Quantification of Cells in Culture / Arleen Richardson, Sergey Fedoroff 333
- 27 Tips for Tissue Culture / Arleen Richardson, Sergey Fedoroff 341.
- Notes:
- Includes bibliographical references and index.
- Local Notes:
- Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.
- ISBN:
- 0896039021
- OCLC:
- 44678489
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