Cellular and molecular methods in neuroscience research / Adalberto Merighi, Giorgio Carmignoto, editors.

Format:

Book

Contributor:

Merighi, Adalberto.

Carmignoto, Giorgio.

Language:

English

Subjects (All):

Molecular neurobiology--Laboratory manuals.

Molecular neurobiology.

Neurons--Laboratory manuals.

Neurons.

Neurobiology--methods.

Medical Subjects:

Neurobiology--methods.

Genre:

Laboratory manuals.

Physical Description:

xiv, 303 pages, A15 pages of plates : illustrations (some color) ; 27 cm

Place of Publication:

New York : Springer, [2002]

Summary:

Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. The "crosstalk," as the editors call it, between researchers with different backgrounds, that is, histologists, cell and molecular biologists, and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond.

The general idea beyond this project was to put together the contributions from a number of well-known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. This book explains in a clear consistent manner some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field.

The format is uniform: "Overview," "Background," "Protocols," and "Results and Discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, a step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four-color plates) to make the concepts and procedures easy to understand and perform.

Contents:

1 Analyses of Intracellular Signal Transduction Pathways in CNS Progenitor Cells / Elena Cattaneo, Luciano Conti 1

2 Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors / Alain Beaudet, Alexander C. Jackson, Franck Vandenbulcke 15

3 Transfection Methods for Neurons in Primary Culture / Christoph Kaether, Martin Kohrmann, Carlos G. Dotti, Francesca Ruberti 29

4 Polyethylenimine: a Versatile Cationic Polymer for Plasmid-Based Gene Delivery in the CNS / Barbara A. Demeneix, Gregory F. Lemkine, Hajer Guissouma 37

5 Transfection of GABA[subscript A] Receptor with GFP-Tagged Subunits in Neurons and HEK 293 Cells / Stefano Vicini, Jin Hong Li, Wei Jian Zhu, Karl Krueger, Jian Feng Wang 53

6 Neuronal Transfection Using Particle-Mediated Gene Transfer / Harold Gainer, Raymond L. Fields, Shirley B. House 67

7 Analysis of Gene Expression in Genetically Labeled Single Cells / Stefano Gustincich, Andreas Feigenspan, Elio Raviola 85

8 Immunocytochemistry and In Situ Hybridization: Their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons / Marc Landry, Andre Calas 119

9 In Situ Reverse Transcription PCR for Detection of mRNA in the CNS / Helle Broholm, Steen Gammeltoft 145

10 Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity / Patrizia Aimar, Laura Lossi, Adalberto Merighi 161

11 Combined Electrophysiological and Morphological Analyses of CNS Neurons / Alfredo Ribeiro-da-Silva, Yves De Koninck 181

12 Tract Tracing Methods at the Light Microscopic Level / Marina Bentivoglio, Giuseppe Bertini 203

13 Tract Tracing Methods at the Ultrastructural Level / Isaura Tavares, Armando Almeida, Deolinda Lima 221

14 In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS / Laura Lossi, Silvia Mioletti, Patrizia Aimar, Renato Bruno, Adalberto Merighi 235

15 Confocal Imaging of Nerve Cells and Their Connections / Andrew J. Todd 259

16 Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices / Wim Scheenen, Giorgio Carmignoto 273.

Notes:

Includes bibliographical references and index.

ISBN:

0387953868

OCLC:

48170934