Viral vectors for gene therapy : methods and protocols / edited by Curtis A. Machida.

Format:

Book

Contributor:

Machida, Curtis A.

Series:

Methods in molecular medicine ; 76.

Methods in molecular medicine ; 76

Language:

English

Subjects (All):

Gene therapy--Laboratory manuals.

Gene therapy.

Genetic vectors--Laboratory manuals.

Genetic vectors.

Transfection--Laboratory manuals.

Transfection.

Viral genetics--Laboratory manuals.

Viral genetics.

Genetic Vectors.

Genetic Therapy.

Gene Transfer Techniques.

Viruses.

Medical Subjects:

Genetic Vectors.

Genetic Therapy.

Gene Transfer Techniques.

Viruses.

Genre:

Laboratory manuals.

Physical Description:

xv, 589 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2003]

Summary:

The promise of gene therapy can be realized only if workable vectors can be found to deliver therapeutic genes. In Viral Vectors for Gene Therapy: Methods and Protocols, leading researchers from academia and biotechnology describe proven molecular methods for the construction, development, and use of virus vectors for gene transfer and gene therapy. Offering detailed step-by-step instructions to ensure successful results, these experts detail the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Additional chapters demonstrate the use of virus vectors in the brain and central nervous system. Each protocol includes a discussion of the principles involved, numerous charts and tables, ample references, and notes on possible problems, troubleshooting, and alternative procedures.

Comprehensive and highly practical, Viral Vectors for Gene Therapy: Methods and Protocols provides not only researchers with the basic tools needed to design targeted gene delivery vectors, but also clinicians with an understanding of how to apply viral vectors to the treatment of genetic disorders.

Contents:

1 Use of the Herpes Simplex Viral Genome to Construct Gene Therapy Vectors / Edward A. Burton, Shaohua Huang, William F. Goins, Joseph C. Glorioso 1

2 Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous System / Caroline E. Lilley, Robert S. Coffin 33

3 Improved HSV-1 Amplicon Packaging System Using ICP27-Deleted, Oversized HSV-1 BAC DNA / Yoshinaga Saeki, Xandra O. Breakefield, E. Antonio Chiocca 51

4 Herpes Simplex Amplicon Vectors / Charles J. Link, Nicholas N. Vahanian, Suming Wang 61

5 Strategies to Adapt Adenoviral Vectors for Targeted Delivery / Catherine R. O'Riordan, Antonius Song, Julia Lanciotti 89

6 Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain: Evaluation of Gene Transfer Efficiency, Toxicity, and Inflammatory and Immune Reactions / Andres Hurtado-Lorenzo, Anne David, Clare Thomas, Maria G. Castro, Pedro R. Lowenstein 113

7 Generation of Adenovirus Vectors Devoid of All Virus Genes by Recombination Between Inverted Repeats / Hartmut Stecher, Cheryl A. Carlson, Dmitry M. Shayakhmetov, Andre Lieber 135

8 Packaging Cell Lines for Generating Replication-Defective and Gutted Adenoviral Vectors / Jeffrey S. Chamberlain, Catherine Barjot, Jeannine Scott 153

9 Improving the Transcriptional Regulation of Genes Delivered by Adenovirus Vectors / Semyon Rubinchik, Jan Woraratanadharm, Jennifer Schepp, Jian-yun Dong 167

10 Targeted Integration by Adeno-Associated Virus / Matthew D. Weitzman, Samuel M. Young, Jr., Toni Cathomen, Richard Jude Samulski 201

11 Development and Optimization of Adeno-Associated Virus Vector Transfer into the Central Nervous System / Matthew J. During, Deborah Young, Kristin Baer, Patricia Lawlor, Matthias Klugmann 221

12 A Method for Helper Virus-Free Production of Adeno-Associated Virus Vectors / Roy F. Collaco, James P. Trempe 237

13 Novel Tools for Production and Purification of Recombinant Adeno-Associated Viral Vectors / Julian D. Harris, Stuart G. Beattie, J. George Dickson 255

14 Recombinant Adeno-Associated Viral Vector Types 4 and 5: Preparation and Application for CNS Gene Transfer / Beverly L. Davidson, John A. Chiorini 269

15 Trans-Splicing Vectors Expand the Packaging Limits of Adeno-Associated Virus for Gene Therapy Applications / Dongsheng Duan, Yongping Yue, Ziying Yan, John F. Engelhardt 287

16 Generation of Retroviral Packaging and Producer Cell Lines for Large-Scale Vector Production with Improved Safety and Titer / Thomas W. Dubensky, Jr., Sybille L. Sauter 309

17 An Ecdysone-Inducible Expression System for Use with Retroviruses / Karen Morse, John Olsen 331

18 In Vivo Infection of Mice by Replication-Competent MLV-Based Retroviral Vectors / Estanislao Bachrach, Mogens Duch, Mireia Pelegrin, Hanna Dreja, Finn Skou Pedersen, Marc Piechaczyk 343

19 Development of Simian Retroviral Vectors for Gene Delivery / Biao Li, Curtis A. Machida 353

20 Self-Inactivating Lentiviral Vectors and a Sensitive Cre-loxP Reporter System / Lung-Ji Chang, Anne-Kathrin Zaiss 367

21 Lentiviral Vectors for Gene Transfer to the Central Nervous System: Applications in Lysosomal Storage Disease Animal Models / Deborah J. Watson, John H. Wolfe 383

22 A Highly Efficient Gene Delivery System Derived from Feline Immunodeficiency Virus (FIV) / Sybille L. Sauter, Medhi Gasmi, Thomas W. Dubensky, Jr. 405

23 A Multigene Lentiviral Vector System Based on Differential Splicing / Yonghong Zhu, Vicente Planelles 433

24 Production of Trans-Lentiviral Vector with Predictable Safety / John C. Kappes, Xiaoyun Wu, John K. Wakefield 449

25 Human Immunodeficiency Virus Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells / Ali Ramezani, Robert G. Hawley 467

26 Semliki Forest Viral Vectors for Gene Transfer / Jarmo Wahlfors, Richard A. Morgan 493

27 Semliki Forest Virus (SFV) Vectors in Neurobiology and Gene Therapy / Kenneth Lundstrom, Markus U. Ehrengruber 503

28 Semliki Forest Virus Vectors for Large-Scale Production of Recombinant Proteins / Kenneth Lundstrom 525

29 Development of Foamy Virus Vectors / George Vassilopoulos, Neil C. Josephson, Grant Trobridge 545

30 Poxviral/Retroviral Chimeric Vectors Allow Cytoplasmic Production of Transducing Defective Retroviral Particles / Georg W. Holzer, Falko G. Falkner 565.

Notes:

Includes bibliographical references and index.

ISBN:

1588290190

OCLC:

50080008