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Structural determination of DNA using residual dipolar couplings / Douglas MacDonald.
Chemistry Library - Reading Room QD001 2002 .M135
Available
LIBRA Diss. POPM2002.208
Available from offsite location
- Format:
- Book
- Manuscript
- Microformat
- Thesis/Dissertation
- Author/Creator:
- MacDonald, Douglas A.
- Language:
- English
- Subjects (All):
- Penn dissertations--Chemistry.
- Chemistry--Penn dissertations.
- Local Subjects:
- Penn dissertations--Chemistry.
- Chemistry--Penn dissertations.
- Physical Description:
- xi, 173 pages : illustrations (some color) ; 29 cm
- Production:
- 2002.
- Summary:
- The molecular interactions between proteins and DNA are being categorized at a rapid rate. However the solution structure of the DNA prior to complex formation is still a void. Is the DNA a uniform structure or does the site of action at the DNA and its flanking sequences exhibit a defined structural target? Structural analysis of DNA by x-ray crystallography has been ambiguous. Structural determination by solution NMR spectroscopy has been limited due to the lack of long-range restraints available from the Nuclear Overhauser Effect and J coupling data. Recently a method to obtain long-range angular information in solution NMR spectroscopy was introduced. The measurement of residual dipolar couplings (RDCs) in a slightly orientating solution has the promise to offer a route to determine high-resolution solution structures of DNA sequences that are protein targets.
- We solved the solution structure of an A-tract DNA bend along with a control sequence using RDCs. Unlike several x-ray diffraction structures the solution structures are consistent with all previous twenty years of biochemical experiments on A-tracts. Having demonstrated the validity of RDCs for nucleic acid structural determination we have used these couplings to gather structural information from promoter DNA sequences. Initial results suggest that the Adenovirus Major Late Promoter (AdMLP) has local structural deviations in its TATA box.
- Notes:
- Adviser: Ponzy Lu.
- Thesis (Ph.D. in Chemistry) -- University of Pennsylvania, 2002.
- Includes bibliographical references.
- Local Notes:
- University Microfilms order no.: 3054976.
- OCLC:
- 244972072
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