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Molecular cytogenetics : protocols and applications / edited by Yao-Shan Fan.
Holman Biotech Commons QH506 .M45 v.204 2002
Available
Levy Dental Medicine Library - Stacks QH441.5 .M654 2002
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; v. 204.
- Methods in molecular biology ; v. 204
- Language:
- English
- Subjects (All):
- Cytogenetics--Laboratory manuals.
- Cytogenetics.
- Molecular genetics--Laboratory manuals.
- Molecular genetics.
- Fluorescence in situ hybridization--Laboratory manuals.
- Fluorescence in situ hybridization.
- Molecular Biology.
- In Situ Hybridization, Fluorescence.
- Medical Subjects:
- Cytogenetics.
- Molecular Biology.
- In Situ Hybridization, Fluorescence.
- Genre:
- Laboratory manuals.
- Physical Description:
- xiv, 411 pages : illustrations (some color) ; 24 cm.
- Place of Publication:
- Totowa, N.J. : Humana Press, [2002]
- Summary:
- Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
- Contents:
- Part I Basic Concepts and Techniques
- 1 Molecular Cytogenetics in Medicine: An Overview / Yao-Shan Fan 3
- 2 Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets / Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, Kim A. Wilber 21
- 3 Labeling Fluorescence In Situ Hybridization Probes for RNA Targets / Ramesh Ramakrishnan, Larry E. Morrison 41
- 4 Basic FISH Techniques and Troubleshooting / Sue Van Stedum, Walter King 51
- Part II Evolving Techniques and Applications
- 5 Chromosome Microdissection / Xin-Yuan Guan 67
- 6 Primed In Situ Labeling / Jorn Koch 77
- 7 Spectral Karyotyping / Jane Bayani, Jeremy A. Squire 85
- 8 Multicolor FISH / Syed M. Jalal, Mark E. Law 105
- 9 Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization / Brynn Levy, Kurt Hirschhorn 121
- 10 Color Banding / Vicki L. Hopwood, Christi J. Hada, Armand B. Glassman 133
- 11 Multicolor Fiber FISH / Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap, Philip M. Kluin, Ed Schuuring 143
- 12 Multi-Telomere FISH / Samantha J. L. Knight, Jonathan Flint 155
- 13 Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements / Laurence Colleaux, Solange Heuertz, Florence Molinari, Marlene Rio 181
- 14 Microarray CGH / Ben Beheshti, Paul C. Park, Ilan Braude, Jeremy A. Squire 191
- Part III Special Applications in Chromosomal Disorders
- 15 Molecular Approaches for Delineating Marker Chromosomes / Michael D. Graf, Stuart Schwartz 211
- 16 Interphase FISH for Prenatal Diagnosis of Common Aneuploidies / Baruch Feldman, Ayala Aviram-Goldring, Mark I. Evans 219
- 17 Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood / Farideh Z. Bischoff, Dianne X. Dang, Dorothy E. Lewis, Joe Leigh Simpson 243
- 18 Preimplantation FISH Diagnosis of Aneuploidies / Yury Verlinsky, Jeanine Cieslak, Anver Kuliev 259
- 19 Microdeletion Syndromes: Characteristics and Diagnosis / Stuart Schwartz, Michael D. Graf 275
- 20 Molecular Detection of Uniparental Disomy / Wendy P. Robinson 291
- 21 Molecular Cytogenetics in Reproductive Pathology / Helene Bruyere, Evica Rajcan-Separovic, Dagmar K. Kalousek 299
- Part IV Special Applications in Oncology
- 22 Interphase FISH Studies of Chronic Myeloid Leukemia / Gordon W. Dewald 311
- 23 Chromogenic In Situ Hybridization and FISH in Pathology / Bae-Li Hsi, Sheng Xiao, Jonathan A. Fletcher 343
- 24 FISH Detection of HER2 Amplification in Breast Cancer / Rosemary E. Mueller, Frances P. O'Malley 353
- 25 Comparative Genomic Hybridization in Cancer Investigations / Marjan M. Weiss, Mario A. J. A. Hermsen, Gerrit A. Meijer, Paul J. van Diest 369
- 26 Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells / Yanming Zhang, Brigitte Schlegelberger 379
- 27 BAC Resource for Molecular Cytogenetics / Xiao-Ning Chen, Julie R. Korenberg 391.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 1588290069
- OCLC:
- 49385864
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