Rapid cycle real-time PCR : methods and applications : genetics and oncology / W. Dietmaier, C. Wittwer, N. Sivasubramanian, eds.

Format:

Book

Contributor:

Dietmaier, W. (Wolfgang), 1963-

Wittwer, C. (Carl), 1955-

Sivasubramanian, N. (Natarajan)

Language:

English

Subjects (All):

Polymerase chain reaction.

Molecular genetics--Methodology.

Molecular genetics.

Cancer--Genetic aspects.

Cancer.

Molecular probes.

Molecular Diagnostic Techniques.

Genetic Diseases, Inborn--diagnosis.

Molecular Probes.

Neoplasms--diagnosis.

Polymerase Chain Reaction--methods.

Medical Subjects:

Molecular Diagnostic Techniques.

Genetic Diseases, Inborn--diagnosis.

Molecular Probes.

Neoplasms--diagnosis.

Polymerase Chain Reaction--methods.

Physical Description:

viii, 205 pages : illustrations (some color) ; 25 cm

Place of Publication:

Berlin ; New York : Springer, [2002]

Summary:

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Notes:

Includes bibliographical references.

ISBN:

3540426000

OCLC:

49704950