Drug discovery and evaluation : pharmacological assays / H. Gerhard Vogel (ed.).

Format:

Book

Contributor:

Vogel, H. Gerhard, 1927-

Clarence J. Marshall Memorial Library Fund.

Language:

English

Subjects (All):

Pharmacology, Experimental.

Drugs--Design.

Drugs.

Drugs--Testing.

Drug Design.

Drug Evaluation--methods.

Drug Evaluation, Preclinical--methods.

Medical Subjects:

Drug Design.

Drug Evaluation--methods.

Drug Evaluation, Preclinical--methods.

Physical Description:

xliv, 1408 pages ; 28 cm + 1 CD-ROM (4 3/4 in.)

Edition:

Second completely revised, updated, and enlarged edition.

Place of Publication:

Berlin ; New York : Springer, [2002]

System Details:

System requirements: Windows: Windows 95 or higher; Pentium-processor; 32 MB RAM. Macintosh: Apple Power Macintosh or compatible; MacOs software version 7 or higher. For both: Adobe Acrobat Reader 4.0.

text file

Summary:

Now expanded and updated to include molecular biology and genetic engineering techniques!

The 2nd edition of this successful reference book contains a comprehensive selection of the most frequently used assays for reliably detecting the pharmacological effects of potential drugs. Effects covered include cardiovascular, analgesic, endocrine, psychotropic respiratory, renal and immunomodulatory activities. Each of the more than 1000 assays comprises a detailed protocol outlining the purpose and rationale of the method, a critical assessment of the results and their pharmacological and clinical relevance. The enclosed and fully searchable CD-ROM allows easy identification of specific tests. An appendix with up-to-date guidelines and legal regulations for animal experiments in various countries will help the reader to plan experiments more effectively.

Contents:

0.1 Historical approaches in drug discovery 1

0.2 Pharmacological approaches of modern medicine 2

0.3 New approaches in drug discovery 3

0.4 Errors in screening procedures 20

Chapter A Cardiovascular activity 23

A.1 Cardiovascular analysis 26

A.2 Methods to induce experimental hypertension 172

A.3 Coronary drugs 180

A.4 Calcium uptake inhibiting activity 201

A.5 Anti-arrhythmic activity 209

A.6 Methods to induce cardiac hypertrophy and insufficiency 229

A.7 Positive inotropic activity (cardiac glycosides) 235

A.8 Effects on blood supply and on arterial and venous tonus 242

Chapter B Activity on blood constituents 277

B.1 In vitro tests 277

B.2 In vivo or ex vivo models 289

B.3 Bleeding models 307

B.4 Genetically modified animals 309

Chapter C Activity on urinary tract 317

C.1 Diuretic and saluretic activity 317

C.2 Impaired renal function 328

C.3 Uricosuric and hypo-uricemic activity 337

C.4 Influence on lower urinary tract 342

Chapter D Respiratory activity 351

D.1 In vitro tests 351

D.2 Effects on air ways 352

D.3 Antitussive activity 373

D.4 Effects on tracheal cells and bronchial mucus secretion and transport 376

Chapter E Psychotropic and neurotropic activity 385

E.1 Effects on behavior and muscle coordination 388

E.2 Tests for anxiolytic activity 401

E.3 Anti-epileptic activity 459

E.4 Hypnotic activity 495

E.5 Neuroleptic activity 500

E.6 Antidepressant activity 545

E.7 Anti-Parkinsonism activity 577

E.8 General anesthesia 586

Chapter F Drug effects on learning and memory 595

F.2 In vitro methods 599

F.3 In vivo methods 619

Chapter G Effects on peripheral nerve function 645

G.1 Local anesthetic activity 645

G.2 Neuromuscular blocking activity 663

Chapter H Analgesic, anti-inflammatory, and anti-pyretic activity 669

H.1 Central analgesic activity 670

H.2 Peripheral analgesic activity 716

H.3 Anti-inflammatory activity 725

H.4 Antipyretic activity 772

Chapter I Antiarthrotic and immunmodulatory activity 775

I.1 Anti-arthrotic activity 775

I.2 Methods for testing immunological factors 788

Chapter J Activity on the gastrointestinal tract 825

J.1 Salivary glands 827

J.2 Esophagus 828

J.3 Gastric function 833

J.4 Intestinal functions 873

J.5 Emetic and anti-emetic activity 901

J.6 Gall bladder functions 906

J.7 Pancreatic function 914

J.8 Liver function 936

J.9 Eviscerated animals 945

Chapter K Antidiabetic activity 947

K.1 Methods to induce experimental diabetes mellitus 948

K.2 Genetically diabetic animals 954

K.3 Assays of insulin and of insulin-like activity 965

K.4 Assays of other glucose regulating peptide hormones 1005

K.5 Blood glucose lowering activity of antidiabetic drugs 1016

K.6 Activity in isolated organs, cells, and membranes 1024

K.7 Inhibition of polysaccharide degrading enzymes 1042

K.8 Effect on secondary diabetes symptoms 1044

Chapter L Anti-obesity activity 1053

L.1 Methods to induce experimental obesity 1053

L.2 Genetically obese animals 1058

L.3 Assays of anti-obesity activity 1065

L.4 Assays of obesity regulating peptide hormones 1075

Chapter M Anti-atherosclerotic activity 1095

M.1 Induction of experimental atherosclerosis 1095

M.2 Influence on lipid metabolism 1102

M.3 Inhibition of cholesterol biosynthesis 1111

M.4 Inhibition of cholesterol absorption 1118

M.5 Interruption of bile acid recirculation 1122

M.6 Inhibition of lipid oxidation 1123

Chapter N Endocrinology 1127

N.1 Endocrine survey 1130

N.2 Adrenal steroid hormones 1131

N.3 Ovarian hormones 1154

N.4 Testicular steroid hormones 1172

N.5 Thyroid hormones 1184

N.6 Parathyroid hormone 1194

N.7 Anterior pituitary hormones 1201

N.8 Posterior pituitary hormones 1236

N.9 Hypothalamic hormones 1247

N.10 Other peptide hormones 1274

Chapter O Ophthalmologic activity 1295

O.2 Intraocular pressure 1295

O.3 Aqueous humor flow rate 1299

O.4 Experimental glaucoma 1300

O.5 Local anesthesia of the cornea 1302

O.6 Experimental cataract formation 1302

O.7 Models of eye inflammation 1303

Chapter P Pharmacological models in dermatology 1311

P.1 Skin sensitization testing 1312

P.2 Experimental dermatitis 1314

P.3 Pruritus models 1318

P.4 Psoriasis models 1320

P.5 Scleroderma models 1331

P.6 Pemphigus models 1333

P.7 Ichthyosis vulgaris models 1334

P.8 Xeroderma models 1335

P.9 Acne models 1336

P.10 Skin mycosis 1345

P.11 Biomechanics of skin 1347

P.12 Protection against UV light 1362

P.13 Transepidermal water loss (TEWL) 1364

P.14 Influence on hair growth 1365

P.15 Cutaneous microcirculation 1367

Chapter Q Guidelines for the care and use of laboratory animals 1369

Q.1 Regulations for the care and use of laboratory animals in various countries 1369

Q.2 Techniques of blood collection in laboratory animals 1371

Q.3 Anesthesia of experimental animals 1375

Q.4 Euthanasia of experimental animals 1380.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Clarence J. Marshall Memorial Library Fund.

ISBN:

3540423966

OCLC:

49057631