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GTPase protocols : the Ras superfamily / edited by Ed Manser and Thomas Leung.
Holman Biotech Commons QH506 .M45 v.189 2002
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; v. 189.
- Methods in molecular biology ; v. 189
- Language:
- English
- Subjects (All):
- Guanosine triphosphatase--Laboratory manuals.
- Guanosine triphosphatase.
- Ras proteins--Laboratory manuals.
- Ras proteins.
- Guanosine Triphosphate.
- ras Proteins.
- GTP Phosphohydrolases.
- Medical Subjects:
- Guanosine Triphosphate.
- ras Proteins.
- GTP Phosphohydrolases.
- Genre:
- Laboratory manuals.
- Physical Description:
- xi, 267 pages : illustrations (some color) ; 24 cm.
- Place of Publication:
- Totowa, NJ : Humana Press, 2002.
- Summary:
- Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Presented in a format that ensures ready reproducibility by accomplished experimentalists who have refined the various methods in their laboratories, each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis. Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.
- Contents:
- Part I. General Protocols
- 1 The GTPase Cycle: How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases / Edward J. Manser 3
- 2 Preparation of GTPases for Structural and Biophysical Analysis / Susan J. M. Smith, Katrin Rittinger 13
- 3 Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes / Arndt A. P. Schmitz, Robert Lucito, Linda Van Aelst 25
- 4 Fluorescence Methods in the Study of Small GTP-Binding Proteins / Mohammad Reza Ahmadian, Alfred Wittinghofer, Christian Herrmann 45
- Part II. Specific Methods
- Ras Family GTPases
- 5 Ras and Rac as Activators of Reactive Oxygen Species (ROS) / Herbert Archer, Dafna Bar-Sagi 67
- 6 Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs / Hiroshi Maruta, Hong He, Thao Nheu 75
- 7 A Ras-Based Module to Generate [superscript 32]P-Labeled Fusion Proteins for Blot Overlays / Zhoushen Zhao, Louis Lim 87
- Rho Family GTPases
- 8 Determination of the Activity of Rho-Like GTPases in Cells / Angeliki Malliri, Jean P. ten Klooster, Christina Olivio, John G. Collard 99
- 9 Introduction of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases / Ivan Tan, Louis Lim, Thomas Leung 111
- 10 Effects of Rho Family GTPases on Cell-Cell Adhesion / Masaki Fukata, Masato Nakagawa, Shinya Kuroda, Kozo Kaibuchi 121
- 11 Cell Motility and Invasion Assays / Jackie Banyard, Marc Symons 129
- Rab Family GTPases
- 12 Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases / Hiroyuki Nakanishi, Takuya Sasaki, Yoshimi Takai 143
- 13 Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase / Jose B. Pereira-Leal, Anita Q. Gomes, Miguel C. Seabra 157
- Arf Family GTPases
- 14 Preparation of Myristoylated Arf1 and Arf6 Proteins / Paul A. Randazzo, Henry M. Fales 169
- 15 ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins / Gustavo Pacheco-Rodriguez, Joel Moss, Martha Vaughan 181
- 16 Arf6 and its Role in Cytoskeletal Modulation / Julie G. Donaldson 191
- 17 ARF GTPase-Activating Protein 1 / Irit Huber, Edna Cukierman, Miriam Rotman, Dan Cassel 199
- Ran GTPases
- 18 The Use of Permeabilized Cell Systems to Study Nuclear Transport / Amy M. Brownawell, James M. Holaska, Ian G. Macara, Bryce M. Paschal 209
- 19 Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes / Ralph H. Kehlenbach, Larry Gerace 231
- 20 Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton / Sofia B. Lizarraga, Yixian Zheng, Andrew R. Wilde 247.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 089603934X
- OCLC:
- 49285457
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