Green fluorescent protein : applications and protocols / edited by Barry W. Hicks.

Format:

Book

Contributor:

Hicks, Barry W.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 183.

Methods in molecular biology ; v. 183

Language:

English

Subjects (All):

Green fluorescent protein--Laboratory manuals.

Green fluorescent protein.

Luminescent Proteins.

Fluorescence.

Medical Subjects:

Luminescent Proteins.

Fluorescence.

Genre:

Laboratory manuals.

Physical Description:

xiv, 393 pages : illustrations ; 24 cm + 1 computer optical disc (4 3/4 in.)

Place of Publication:

Totowa, NJ : Humana Press, 2002.

System Details:

System requirements for accompanying disc: Netscape Navigator or Internet Explorer version 4.0 or higher.

text file

Summary:

Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses. A valuable companion CD-ROM displays many color figures and videos from over half the chapters.

Contents:

Part I. Manipulation of Green Fluorescent Protein Structure at the Genetic Level

1 Amplification of Representative cDNA Samples from Microscopic Amounts of Invertebrate Tissue to Search for New Genes / Mikhail V. Matz 3

2 Use of cobA and cysG[superscript A] as Red Fluorescent Indicators / Charles A. Roessner 19

3 Circular Permutation of the Green Fluorescent Protein / Simon Topell, Rudi Glockshuber 31

4 Evolutionary Design of Generic Green Fluorescent Protein Biosensors / Nobuhide Doi, Hiroshi Yanagawa 49

5 Random Insertion of Green Fluorescent Protein into the Regulatory Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase / Pascal J. Baehler, Ricardo M. Biondi, Miguel van Bemmelen, Michel Veron, Christophe D. Reymond 57

6 Circular mRNA Encoding for Monomeric and Polymeric Green Fluorescent Protein / Rhonda Perriman 69

Part II. Detection and Imaging of Green Fluorescent Protein

7 Fluorescence Lifetime Imaging (FLIM) of Green Fluorescent Fusion Proteins in Living Cells / Ammasi Periasamy, Masilamani Elangovan, Elizabeth Elliott, David L. Brautigan 89

8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein: Energy Transfer to the Endogenous Chromophores of Phycobilisome Light-Harvesting Complexes / Jasper J. van Thor, Klaas J. Hellingwerf 101

9 Bioluminescence Resonance Energy Transfer Assays for Protein-Protein Interactions in Living Cells / Yao Xu, Carl Hirschie Johnson, David Piston 121

10 Whole-Body Fluorescence Imaging with Green Fluorescence Protein / Robert M. Hoffman 135

Part III. Green Fluorescent Protein to Monitor Protein Distribution and Trafficking

11 Drug-Induced Translocation of Nucleolar Proteins Fused to Green Fluorescent Protein / Benigno C. Valdez, Laszlo Perlaky 151

12 Light-Induced Nuclear Targeting of PhytochromeB-sGreen Fluorescent Protein in Plants / Akira Nagatani, Tomonao Matsushita 163

13 Mechanisms of Protein Trafficking: Two Different Signal Sequences Fused to Green Fluorescent Protein to Study Mitochondrial Import / Henry Weiner 171

14 Analysis of Nucleocytoplasmic Transport Using Green Fluorescent Protein / Roland H. Stauber 181

Part IV. Green Fluorescent Protein in Transgenic Organisms

15 Transgenic Bovine Embryo Selection Using Green Fluorescent Protein / Anthony W. S. Chan, Kowit-Yu Chong, Gerald Schatten 201

16 Development of Glycosylphosphatidylinositol-Anchored Enhanced Green Fluorescent Protein: One-Step Visaulization of GPI Fate in Global Tissues and Ubiquitous Cell Surface Marking / Gen Kondoh 215

17 Transgenic Zebrafish Expressing Green Fluorescent Protein / Ebrahim Shafizadeh, Haigen Huang, Shuo Lin 225

18 Transgenic Insects: Expressing Green Fluorescent Protein-Silk Fibroin Light Chain Fusion Protein in Transgenic Silkworms / Hajime Mori 235

19 Green Fluorescent Protein in Transgenic Plants: Brassica Transformation / C. Neal Stewart, Jr., Matthew D. Halfhill, Reginald J. Millwood 245

Part V. Green Fluorescent Protein Biosensors

20 Green Fluorescent Protein Calcium Biosensors: Calcium Imaging with GFP Cameleons / Aniko Varadi, Guy A. Rutter 255

21 Green Fluorescent Protein Fluobody Immunosensors: Immunofluorescence with GFP-Antibody Fusion Proteins / Arjen Schots, Jan M. van der Wolf 265

22 Green Fluorescent Protein-Based Protein Kinase Biosensor Substrates / Scott Ulrich, Kevan Shokat 275

23 Green Fluorescent Protein Urea Sensors: Uropathogenic Proteus mirabilis / Christopher Coker, Hui Zhao, Harry L. T. Mobley 287

Part VI. Viral Applications of Green Fluorescent Protein

24 Using Green Fluorescent Protein to Monitor Measles Virus Cell-to-Cell Spread by Time-Lapse Confocal Microscopy / W. Paul Duprex, Bert K. Rima 297

25 Tracking and Selection of Retrovirally Transduced Murine Bone Marrow Cells Using Green Fluorescent Protein / Jessamyn Bagley, John Iacomini 309

26 Green Fluorescent Protein as a Reporter of Adenovirus-Mediated Gene Transfer and Expression in the Hypothalamic-Neurohypophyseal System / Elisardo Corral Vasquez, Alan Kim Johnson 321

27 Enhancement of Green Fluorescent Protein Expression in Adeno-Associated Virus with the Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Element / Jonathan E. Loeb, Matthew D. Weitzman, Thomas J. Hope 331

28 Construction of Infectious Simian Varicella Virus Expressing Green Fluorescent Protein / Ravi Mahalingam, Donald H. Gilden 341

29 Green Fluorescent Protein in Retroviral Vector Constructs as Marker and Reporter of Gene Expression for Cell and Gene Therapy Applications / Nicoletta Elipoulos, Jacques Galipeau 353.

Notes:

Includes bibliographical references and index.

ISBN:

0896039056

OCLC:

63704873